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PMID: 2950137 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Myosin heavy chain messenger RNA and protein isoform transitions during cardiac hypertrophy. Interaction between hemodynamic and thyroid hormone-induced signals.

The Journal of clinical investigation ·Vol. 79 ·No. 3 ·1987-03-00 ·Pages 970-7

Izumo S, Lompré AM, Matsuoka R, Koren G, Schwartz K, Nadal-Ginard B, Mahdavi V

Abstract

Expression of the cardiac myosin isozymes is regulated during development, by hormonal stimuli and hemodynamic load. In this study, the levels of expression of the two isoforms (alpha and beta) of myosin heavy chain (MHC) during cardiac hypertrophy were investigated at the messenger RNA (mRNA) and protein levels. In normal control and sham-operated rats, the alpha-MHC mRNA predominated in the ventricular myocardium. In response to aortic coarctation, there was a rapid induction of the beta-MHC mRNA followed by the appearance of comparable levels of the beta-MHC protein in parallel to an increase in the left ventricular weight. Administration of thyroxine to coarctated animals caused a rapid deinduction of beta-MHC and induction of alpha-MHC, both at the mRNA and protein levels, despite progression of left ventricular hypertrophy. These results suggest that the MHC isozyme transition during hemodynamic overload is mainly regulated by pretranslational mechanisms, and that a complex interplay exists between hemodynamic and hormonal stimuli in MHC gene expression.

MeSH Terms
Animals Aortic Coarctation Cardiomegaly/etiology,genetics,physiopathology Gene Expression Regulation/drug effects Heart Atria/physiopathology Heart Ventricles/physiopathology Hemodynamics Male Myosins/genetics RNA, Messenger/genetics Rats Rats, Inbred Strains Thyroid Hormones/physiology Thyroxine/blood,pharmacology Triiodothyronine/blood
Chemicals
RNA, Messenger Thyroid Hormones Triiodothyronine Myosins Thyroxine
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Izumo S
Lompré A M
Matsuoka R
Koren G
Schwartz K
Nadal-Ginard B
Mahdavi V
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI112908
Published
1987-03-00
Pages
970-7
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC424251
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI112908 PMC Full Text View journal details
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