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PMID: 6193509 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of mRNA sequences for cardiac alpha- and beta-form myosin heavy chains: expression in ventricles of normal, hypothyroid, and thyrotoxic rabbits.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 19 ·1982-10-00 ·Pages 5847-51

Sinha AM, Umeda PK, Kavinsky CJ, Rajamanickam C, Hsu HJ, Jakovcic S, Rabinowitz M

Abstract

We have isolated cDNA clones from thyrotoxic (pMHC alpha) and normal (pMHC beta) adult rabbit hearts. Restriction map analysis and DNA sequence analyses show that, although there is strong homology between overlapping regions of the two clones, they are distinctly different. The two clones exhibited 78-83% homology between the derived amino acid sequences and those determined by direct amino acid sequence analysis of rabbit fast skeletal muscle myosin heavy chains. The clones specify a segment of the myosin heavy chain corresponding to subfragment 2 and the COOH-terminal portions of subfragment 1. Nuclease S1 mapping was used to compare transcription of the two clones with expression of the alpha and beta forms of myosin heavy chains in the ventricles of thyrotoxic, hypothyroid (propylthiouracil-treated), and normal rabbits. Thyrotoxic ventricles contained only pMHC alpha transcripts whereas hypothyroid ventricles contained exclusively pMHC beta transcripts. These data correlate well with the presence of alpha- and beta-form myosin heavy chains. In the normal young adult rabbit, pMHC beta transcripts predominate, agreeing with the known beta form/alpha form ratio of 4:1. We therefore conclude that pMHC alpha and pMHC beta contain sequences of the alpha- and beta-form myosin heavy chain genes, respectively.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Heart Ventricles/metabolism Hyperthyroidism/metabolism Hypothyroidism/metabolism Male Myocardium/metabolism Myosins/genetics Plasmids Poly A/genetics RNA/genetics RNA, Messenger/genetics Rabbits
Chemicals
RNA, Messenger Poly A RNA DNA DNA Restriction Enzymes Myosins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Sinha A M
Umeda P K
Kavinsky C J
Rajamanickam C
Hsu H J
Jakovcic S
Rabinowitz M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.19.5847
Published
1982-10-00
Pages
5847-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347007
Subset
IM
Grants
NHLBI NIH HHS · HL07381 · United States
NHLBI NIH HHS · HL09172 · United States
NHLBI NIH HHS · HL20592 · United States
Databases
GENBANK
J00671, J00672
External Links
PubMed source DOI: 10.1073/pnas.79.19.5847 PMC Full Text View journal details
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