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PMID: 2946641 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Correlation of the virulence of Klebsiella pneumoniae K1 and K2 with the presence of a plasmid encoding aerobactin.

Infection and immunity ·Vol. 54 ·No. 3 ·1986-12-00 ·Pages 603-8

Nassif X, Sansonetti PJ

Abstract

Nine isolates of Klebsiella pneumoniae belonging to capsular serotypes K1 and K2 were assayed for virulence in mice. Virulent isolates (50% lethal dose of less than 10(3) microorganisms) and avirulent isolates (50% lethal dose of over 10(6) microorganisms) were selected. Supplementation of a defined minimal medium with transferrin markedly reduced the growth of avirulent strains but had no significant effect on the growth of virulent strains. All isolates produced enterochelin, but only production of aerobactin could be correlated with virulence. The genes encoding aerobactin and its receptor protein were located on a 180-kilobase plasmid. They were cloned into the mobilizable vector pSUP202. Homology was demonstrated with the aerobactin operon of the Escherichia coli plasmid pColV-K30. Transfer of the recombinant plasmid pKP4 into an avirulent recipient enhanced virulence by 100-fold. These experiments demonstrated that aerobactin is an essential factor of pathogenicity in K. pneumoniae.

MeSH Terms
Bacterial Outer Membrane Proteins DNA, Bacterial/genetics Genes, Bacterial Hydroxamic Acids/metabolism Iron Chelating Agents/biosynthesis Klebsiella pneumoniae/genetics,pathogenicity Plasmids Receptors, Immunologic/biosynthesis,genetics Siderophores
Chemicals
Bacterial Outer Membrane Proteins DNA, Bacterial Hydroxamic Acids Iron Chelating Agents Receptors, Immunologic Siderophores aerobactin receptor aerobactin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nassif X
Sansonetti P J
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26 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.54.3.603-608.1986
Published
1986-12-00
Pages
603-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260211
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.54.3.603-608.1986 PMC Full Text View journal details
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