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PMID: 4037784 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Plasmid-determined cloacin DF13-susceptibility in Enterobacter cloacae and Klebsiella edwardsii; identification of the cloacin DF13/aerobactin outer membrane receptor proteins.

Antonie van Leeuwenhoek ·Vol. 51 ·No. 2 ·1985-00-00 ·Pages 203-18

Krone WJ, Koningstein G, de Graaf FK, Oudega B

Abstract

Both Enterobacter cloacae H478 and Klebsiella edwardsii S15 were shown to harbour a relatively large conjugative plasmid that coded for cloacin DF13-susceptibility and the production and uptake of a hydroxamate iron chelator, most probably aerobactin. Protein-blotting experiments with antiserum raised against the purified cloacin DF13/aerobactin receptor protein from Escherichia coli (Co1V-K30) revealed that the corresponding outer membrane receptor proteins of Ent. cloacae H478 and K. edwardsii S15 had apparent mol wts of 85 000 and 76 000, respectively. E. coli transconjugants harbouring either the plasmid from Ent. cloacae H478 or K. edwardsii S15 expressed a cloacin DF13/aerobactin outer membrane receptor protein with a mol wt of 74 000. The receptor protein encoded by the Ent. cloacae and K. edwardsii plasmids were immunologically more related to each other than to the pCo1V-K30-encoded receptor protein.

MeSH Terms
Bacterial Outer Membrane Proteins Cloacin/pharmacology Conjugation, Genetic Enterobacter/genetics,metabolism Enterobacteriaceae/genetics Hydroxamic Acids/metabolism Iron Chelating Agents/metabolism Klebsiella/genetics,metabolism Molecular Weight Plasmids Receptors, Immunologic/genetics,immunology
Chemicals
Bacterial Outer Membrane Proteins Hydroxamic Acids Iron Chelating Agents Receptors, Immunologic aerobactin receptor aerobactin Cloacin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Krone W J
Koningstein G
de Graaf F K
Oudega B
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31 references, click to expand
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Article Info
Journal
Antonie van Leeuwenhoek
Abbr.
Antonie Van Leeuwenhoek
ISSN
0003-6072
DOI
10.1007/BF02310013
Published
1985-00-00
Pages
203-18
Language
English
Region
Netherlands
NLM ID
0372625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02310013 View journal details
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