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PMID: 292306 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regional localization of human gene loci on chromosome 9: studies of somatic cell hybrids containing human translocations.

American journal of human genetics ·Vol. 31 ·No. 5 ·1979-09-00 ·Pages 586-600

Mohandas T, Sparkes RS, Sparkes MC, Shulkin JD, Toomey KE, Funderburk SJ

Abstract

Somatic cell hybrids were derived from the fusion of (1) Chinese hamster cells deficient in hypoxanthine guanine phosphoribosyltransferase (HPRT) and human cells carrying an X/9 translocation and (2) Chinese hamster cells deficient in thymidine kinase (TK) and human cells carrying a 17/9 translocation. Several independent primary hybrid clones from these two series of cell hybrids were analyzed cytogenitically for human chromosome content and electrophoretically for the expression of human markers known to be on human chromosome 9. The results allow the assignment of the loci for the enzymes galactose-1-phosphate uridyltransferase (GALT), soluble aconitase (ACONs), and adenylate kinase-3 (AK3) to the short arm of chromosome 9 (p11 to pter) and the locus for the enzyme adenylate kinase-1 (AK1) to the distal end of the long arm of human chromosome 9 (hand q34). Earlier family studies have shown that the locus for AK1 is closely linked to the ABO blood group locus and to the locus of the nail-patella (Np) syndrome. Thus the regional localization of AK1 locus permits the localization of the AK1-Np-ABO linkage group.

MeSH Terms
Aconitate Hydratase/genetics Adenylate Kinase/genetics Animals Chromosome Mapping Chromosomes, Human, 16-18 Chromosomes, Human, 6-12 and X Cricetinae Female Genetic Linkage Genetic Markers Humans Hybrid Cells Translocation, Genetic UTP-Hexose-1-Phosphate Uridylyltransferase/genetics X Chromosome
Chemicals
Genetic Markers Adenylate Kinase UTP-Hexose-1-Phosphate Uridylyltransferase Aconitate Hydratase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mohandas T
Sparkes R S
Sparkes M C
Shulkin J D
Toomey K E
Funderburk S J
References (28)
28 references, click to expand
  1. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.
    Science. 1964 Aug 14;145(3633):709-10 PMID: 14168277
  2. Genetical linkage between the ABO and nail-patella loci.
    Ann Hum Genet. 1955 Jun;19(4):312-31 PMID: 14388536
  3. Improved technique for electrophoresis of human galactose-1-p uridyl transferase (EC 2.7.7.12).
    Hum Genet. 1977 Dec 29;40(1):93-7 PMID: 604249
  4. Assignment of the human gene for galactose-1-phosphate uridyltransferase to chromosome 9: studies with Chinese hamster-human somatic cell hybrids.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5628-31 PMID: 271990
  5. Family studies with the chromosome 9 markers ABO, AK1, ACONs and 9qh.
    Ann Hum Genet. 1977 Jul;41(1):53-60 PMID: 200168
  6. Segregation of ABO, AK1 and ACONs in families with abnormalities of chromosome 9.
    Ann Hum Genet. 1978 Jan;41(3):365-77 PMID: 204246
  7. Mapping AK1, ACONs, and AK3 to chromosome 9 in man employing and X/9 translocation and somatic cell hybrids.
    Cytogenet Cell Genet. 1977;19(1):26-37 PMID: 196813
  8. Localisation of the human ABO: Np-1: AK-1 linkage group by regional assignment of AK-1 to 9q34.
    Hum Genet. 1976 Sep 10;34(1):35-43 PMID: 184030
  9. Assignment of the ABO-Np-AK1 linkage group to chromosome 9 in man-hamster hybrids.
    Cytogenet Cell Genet. 1976;16(1-5):241-3 PMID: 185018
  10. Mental retardation associated with "balanced" chromosome rearrangements.
    Am J Hum Genet. 1977 Mar;29(2):136-41 PMID: 848489
  11. Aconitase polymorphism in man.
    Ann Hum Genet. 1975 Oct;39(2):193-202 PMID: 1052766
  12. Localization and induction of the human thymidine kinase gene by adenovirus 12.
    Nat New Biol. 1973 Oct 10;245(145):172-5 PMID: 4126392
  13. Cellulose acetate electrophoresis of human glucose-6-phosphate dehydrogenase.
    J Lab Clin Med. 1969 Mar;73(3):531-4 PMID: 4179685
  14. Chromosome assignment of the human gene for galactose-1-phosphate uridyltransferase.
    Proc Natl Acad Sci U S A. 1974 Feb;71(2):404-7 PMID: 4521812
  15. Assignment of nucleoside phosphorylase to D-14 and localization of X-linked loci in man by somatic cell genetics.
    Nat New Biol. 1973 Feb 7;241(110):180-2 PMID: 4512579
  16. Assignment of the human gene for hexose-1-phosphate uridylyltransferase to chromosome 3.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3483-6 PMID: 4139713
  17. Adenylate kinases in man: evidence for a third locus.
    Ann Hum Genet. 1976 Jan;39(3):305-13 PMID: 5940
  18. Evidence for the assignment of the loci AK1, AK3 and ACONs to chromosome 9 in man.
    Ann Hum Genet. 1976 May;39(4):413-22 PMID: 182062
  19. Assignment of the AK1:Np:ABO linkage group to human chromosome 9.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):895-9 PMID: 176661
  20. Characteristics of an HPRT-deficient chinese hamster cell line.
    Cytogenet Cell Genet. 1974;13(5):437-47 PMID: 4462979
  21. Parasexual approaches to the genetics of man.
    Annu Rev Genet. 1975;9:407-86 PMID: 1108769
  22. Human X-autosome translocations: differential inactivation of the X chromosome in a kindred with an X-9 translocation.
    Am J Hum Genet. 1975 Jul;27(4):441-53 PMID: 1155455
  23. Differential binding of alkylating fluorochromes in human chromosomes.
    Exp Cell Res. 1970 Jun;60(3):315-9 PMID: 5422961
  24. Electrophoresis of phosphoglycerate kinase.
    Biochem Genet. 1969 Apr;3(2):189-95 PMID: 5364926
  25. Linkage of the loci for the nail-patella syndrome and adenylate kinase.
    Am J Hum Genet. 1969 Nov;21(6):606-30 PMID: 5365763
  26. The genetic linkage relations of adenylate kinase: further data on the ABO-AK linkage group.
    Am J Hum Genet. 1969 Nov;21(6):600-5 PMID: 5365762
  27. Data on the incidence, segregation and linkage relations of the adenylate kinase (AK) polymorphism.
    Ann Hum Genet. 1968 Jan;31(3):237-42 PMID: 5648746
  28. Genetically determined variation of adenylate kinase in man.
    Nature. 1966 Jan 15;209(5020):261-3 PMID: 5915956
Article Info
Journal
American journal of human genetics
Abbr.
Am J Hum Genet
ISSN
0002-9297
Published
1979-09-00
Pages
586-600
Language
English
Region
United States
NLM ID
0370475
PMCID
PMC1685904
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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