Assignment of the human gene for hexose-1-phosphate uridylyltransferase to chromosome 3.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 9 ·1974-09-00 ·Pages 3483-6

Tedesco TA, Diamond R, Orkwiszewski KG, Boedecker HJ, Croce CM

Abstract

Mouse-human hybrid clones were tested for the presence of human hexose-1-phosphate uridylyl-transferase (EC 2.7.7.12;UDPglucose:alpha-D-galactose-1-phosphate uridylyltransferase). Two criteria, starch gel electrophoresis and double-immunodiffusion against a human transferase-specific antibody, were used to identify human enzyme in the hybrid clones. Seventeen of 33 hybrid clones analyzed were found to contain human transferase by both criteria. Karyological analysis of the hybrid clones showed concordant segregation of human transferase with human chromosome 3. Human galactokinase was asyntenic with human transferase. We thus assign this gene to human chromosome 3.

MeSH Terms

Animals Cell Line Chromosome Mapping Chromosomes, Human, 1-3 Electrophoresis, Starch Gel Epitopes Genes Humans Hybrid Cells Immunodiffusion Karyotyping Mice Nucleotidyltransferases/biosynthesis,immunology Phosphotransferases Thymidine Kinase/deficiency Uracil Nucleotides

Chemicals

Epitopes Uracil Nucleotides Phosphotransferases Thymidine Kinase Nucleotidyltransferases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Tedesco T A

Diamond R

Orkwiszewski K G

Boedecker H J

Croce C M

References (12)

12 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.9.3483

Published

1974-09-00

Pages

3483-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433798

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.9.3483 PMC Full Text View journal details

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Assignment of the human gene for hexose-1-phosphate uridylyltransferase to chromosome 3.

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