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PMID: 2922261 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human dopamine beta-hydroxylase gene: two mRNA types having different 3'-terminal regions are produced through alternative polyadenylation.

Nucleic acids research ·Vol. 17 ·No. 3 ·1989-02-11 ·Pages 1089-102

Kobayashi K, Kurosawa Y, Fujita K, Nagatsu T

Abstract

Two kinds of cDNA (types A and B) encoding human dopamine beta-hydroxylase (DBH) were isolated from a pheochromocytoma cDNA library. Type A (2.7 kb) and B (2.4 kb) encoded the same amino acid sequence and were different only in 3'-untranslated region. Type A contained 3'-extension of 300 bp at the end of type B. Subsequently, we isolated human DBH gene and analyzed genomic DNA by Southern hybridization. Human DBH gene (approximately 23 kb) was composed of 12 exons and existed as a single gene on genome. Exon 12 encoded 3'-terminal region of 1,013 bp of type A, including the 300 bp sequence. These results indicate that alternative use of two polyadenylation sites from a single DBH gene generates different mRNA types. This conclusion was supported by Northern hybridization and S1 nuclease mapping experiments. The ratio of type A and B mRNAs in pheochromocytoma was roughly 1.0 to 0.2. We found possible transcription regulatory elements, TATA, CCAAT, CACCC, GC boxes, near the transcription initiation site of DBH gene. Sequences homologous to glucocorticoid and cyclic AMP response elements were also observed.

MeSH Terms
Amino Acid Sequence Base Composition Base Sequence Cloning, Molecular DNA/isolation & purification Dopamine beta-Hydroxylase/biosynthesis,genetics,isolation & purification Genes Humans Molecular Sequence Data Poly A/metabolism Promoter Regions, Genetic RNA, Messenger/biosynthesis,isolation & purification
Chemicals
RNA, Messenger Poly A DNA Dopamine beta-Hydroxylase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kobayashi K
Department of Biochemistry, Nagoya University School of Medicine, Japan.
Kurosawa Y
Fujita K
Nagatsu T
References (41)
41 references, click to expand
  1. Selective induction by nerve growth factor of tyrosine hydroxylase and dopamine- -hydroxylase in the rat superior cervical ganglia.
    Proc Natl Acad Sci U S A. 1971 Jul;68(7):1598-602 PMID: 5283951
  2. Dopamine beta-hydroxylase of adrenal chromaffin granules: structure and function.
    Annu Rev Biochem. 1988;57:551-92 PMID: 3052283
  3. Regulation of dopamine beta-hydroxylase in rat adrenal glands.
    J Biol Chem. 1975 Apr 25;250(8):3204-11 PMID: 235542
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. Selective induction of tyrosine hydroxylase and dopamine beta-hydroxylase in sympathetic ganglia in organ culture: role of glucocorticoids as modulators.
    Mol Pharmacol. 1976 May;12(3):353-61 PMID: 6895
  6. Regulation of rat adrenal dopamine beta-hydroxylase. II. Receptor interaction in the regulation of enzyme synthesis and degradation.
    Brain Res. 1976 Aug 27;113(2):349-62 PMID: 182323
  7. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  8. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  9. Appearance of catecholamine-synthesizing enzymes during development of rat sympathetic nervous system: possible role of tissue environment.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):509-13 PMID: 34153
  10. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  11. The structure and evolution of the human beta-globin gene family.
    Cell. 1980 Oct;21(3):653-68 PMID: 6985477
  12. Multiple polyadenylation sites in a mouse alpha-amylase gene.
    Nucleic Acids Res. 1981 May 25;9(10):2313-23 PMID: 6166922
  13. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  14. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  15. Purification and properties of bovine brain dopamine beta-hydroxylase.
    J Neurochem. 1982 Oct;39(4):1066-71 PMID: 7119782
  16. Efficient isolation of genes by using antibody probes.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
  17. Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit beta-globin gene in mouse 3T6 cells.
    Cell. 1983 Mar;32(3):695-706 PMID: 6299573
  18. Multiple mRNA species with distinct 3' termini are transcribed from the beta 2-microglobulin gene.
    Nature. 1983 Mar 31-Apr 6;302(5907):449-52 PMID: 6188058
  19. Sequence and structure of the coding region of the human H-ras-1 gene from T24 bladder carcinoma cells.
    J Mol Appl Genet. 1983;2(2):173-80 PMID: 6308118
  20. Growth-dependent expression of dihydrofolate reductase mRNA from modular cDNA genes.
    Mol Cell Biol. 1983 Sep;3(9):1598-608 PMID: 6138708
  21. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  22. Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene.
    Nature. 1984 Apr 5-11;308(5959):513-9 PMID: 6323998
  23. Are U4 small nuclear ribonucleoproteins involved in polyadenylation?
    Nature. 1984 May 10-16;309(5964):179-82 PMID: 6325940
  24. Nerve growth factor-mediated enzyme induction in primary cultures of bovine adrenal chromaffin cells: specificity and level of regulation.
    J Neurosci. 1984 Jul;4(7):1771-80 PMID: 6145760
  25. Mutationally altered 3' ends of yeast CYC1 mRNA affect transcript stability and translational efficiency.
    J Mol Biol. 1984 Jul 25;177(1):107-35 PMID: 6086937
  26. Role of the conserved AAUAAA sequence: four AAUAAA point mutants prevent messenger RNA 3' end formation.
    Science. 1984 Nov 30;226(4678):1045-51 PMID: 6208611
  27. Transcription termination and 3' processing: the end is in site!
    Cell. 1985 Jun;41(2):349-59 PMID: 2580642
  28. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  29. Human N-ras: cDNA cloning and gene structure.
    Nucleic Acids Res. 1985 Jul 25;13(14):5255-68 PMID: 2991860
  30. Transcription factor Sp1 recognizes promoter sequences from the monkey genome that are simian virus 40 promoter.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):4915-9 PMID: 2991898
  31. Transient accumulation of c-fos RNA following serum stimulation requires a conserved 5' element and c-fos 3' sequences.
    Cell. 1985 Oct;42(3):889-902 PMID: 2414012
  32. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  33. Reserpine and the monoaminergic regulation of adrenal dopamine beta-hydroxylase activity.
    Neuroscience. 1986;17(1):235-45 PMID: 2938024
  34. Tissue-specific heterogeneity of the 3'-untranslated region of L-type pyruvate kinase mRNAs.
    Eur J Biochem. 1986 Jul 1;158(1):33-41 PMID: 2874025
  35. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
    Cell. 1986 Aug 29;46(5):659-67 PMID: 3488815
  36. Congenital dopamine-beta-hydroxylase deficiency. A novel orthostatic syndrome.
    Lancet. 1987 Jan 24;1(8526):183-8 PMID: 2880016
  37. Cooperativity of glucocorticoid response elements located far upstream of the tyrosine aminotransferase gene.
    Cell. 1987 Apr 10;49(1):29-38 PMID: 2881624
  38. Isolation of a novel cDNA clone for human tyrosine hydroxylase: alternative RNA splicing produces four kinds of mRNA from a single gene.
    Biochem Biophys Res Commun. 1987 Aug 14;146(3):971-5 PMID: 2887169
  39. The primary structure of human dopamine-beta-hydroxylase: insights into the relationship between the soluble and the membrane-bound forms of the enzyme.
    EMBO J. 1987 Dec 20;6(13):3931-7 PMID: 3443096
  40. Cyclic AMP and the induction of eukaryotic gene transcription.
    J Biol Chem. 1988 Jul 5;263(19):9063-6 PMID: 2837474
  41. Acetylcholine-induced stimulation of catecholamine recovery in denervated rat adrenals after reserpine-induced depletion.
    Mol Pharmacol. 1971 Jul;7(4):389-96 PMID: 4398814
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.3.1089
Published
1989-02-11
Pages
1089-102
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331724
Subset
IM
Databases
GENBANK
X13255, X13256, X13257, X13258, X13259, X13260, X13261, X13262, X13263, X13264, X13265, X13266, X13267, X13268
External Links
PubMed source DOI: 10.1093/nar/17.3.1089 PMC Full Text View journal details
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