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PMID: 2909489 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Biological and immunological properties of the carboxyl terminus of staphylococcal enterotoxin C1.

Infection and immunity ·Vol. 57 ·No. 1 ·1989-01-00 ·Pages 23-8

Bohach GA, Handley JP, Schlievert PM

Abstract

Comparisons of recently published primary sequences of staphylococcal and streptococcal pyrogenic toxins prompted an evaluation of biological and immunological properties of the C terminus of staphylococcal enterotoxin C1. The 59 N-terminal amino acids were deleted from the toxin by digestion with trypsin. The resulting fragment (Mr, 20,659) contained the remaining 180 C-terminal residues. This fragment (Trp F1) consisted of two polypeptide chains (Trp F1a and Trp F1b) linked by cysteine residues. Trp F1 was mitogenic, pyrogenic, and enhanced susceptibility of rabbits to lethal endotoxin shock. In addition, this fragment contained at least one antigenic epitope that cross-reacted with enterotoxin B.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Bacterial/immunology Antibodies, Monoclonal/immunology Antigens, Bacterial/immunology Cross Reactions Enterotoxins/immunology,isolation & purification,physiology Mice Mice, Inbred BALB C Mitogens/physiology Molecular Sequence Data Neutralization Tests Peptide Fragments/immunology,isolation & purification,physiology Peptide Mapping Rabbits Staphylococcus aureus/immunology Trypsin
Chemicals
Antibodies, Bacterial Antibodies, Monoclonal Antigens, Bacterial Enterotoxins Mitogens Peptide Fragments enterotoxin C, staphylococcal Trypsin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bohach G A
Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455.
Handley J P
Schlievert P M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.1.23-28.1989
Published
1989-01-00
Pages
23-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313035
Subset
IM
Grants
NHLBI NIH HHS · HI36611 · United States
External Links
PubMed source DOI: 10.1128/iai.57.1.23-28.1989 PMC Full Text View journal details
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