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PMID: 2836707 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and characterization of the gene, speC, for pyrogenic exotoxin type C from Streptococcus pyogenes.

Molecular & general genetics : MGG ·Vol. 212 ·No. 1 ·1988-04-00 ·Pages 66-70

Goshorn SC, Bohach GA, Schlievert PM

Abstract

The structural gene of streptococcal pyrogenic exotoxin type C (SPE C) was cloned from the chromosome of Streptococcus pyogenes strain T18P into Escherichia coli using pBR328 as the vector plasmid. Subcloning enabled the localization of the gene (speC) to a 1.7 kb fragment. Partially purified E. coli-derived SPE C and purified streptococcal-derived SPE C, were shown to have the same molecular weight (23,800) and biological activities. A DNA probe, prepared from cloned speC, cross-hybridized with the structural genes of SPE A and SPE B indicating relatedness at the nucleotide level. The speC-derived probe also hybridized to a fragment of CS112 bacteriophage DNA containing the phage attachment site.

MeSH Terms
Bacterial Proteins Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics Exotoxins/genetics,isolation & purification Genes Genes, Bacterial Membrane Proteins Plasmids Pyrogens Streptococcus pyogenes/genetics
Chemicals
Bacterial Proteins Exotoxins Membrane Proteins Pyrogens SpeA protein, Streptococcus pyogenes erythrogenic toxin DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Goshorn S C
University of Minnesota Medical School, Department of Microbiology, Minneapolis 55455.
Bohach G A
Schlievert P M
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00322445
Published
1988-04-00
Pages
66-70
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NCI NIH HHS · 5T32 CA09138 · United States
NHLBI NIH HHS · HL36611 · United States
External Links
PubMed source DOI: 10.1007/BF00322445
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