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PMID: 2907479 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and sequence-specific DNA binding of the Antennapedia homeodomain.

The EMBO journal ·Vol. 7 ·No. 13 ·1988-12-20 ·Pages 4299-304

Müller M, Affolter M, Leupin W, Otting G, Wüthrich K, Gehring WJ

Abstract

The homeodomain encoded by the Antennapedia (Antp) gene of Drosophila was overproduced in a T7 expression vector in Escherichia coli. The corresponding polypeptide of 68 amino acids was purified to homogeneity. The homeodomain was analysed by ultracentrifugation and assayed for DNA binding. The secondary structure of the isolated homeodomain was determined by nuclear magnetic resonance spectroscopy. DNA-binding studies indicate that the isolated homeodomain binds to DNA in vitro. It selectively binds to the same sites as a longer Antp polypeptide and a full-length fushi tarazu (ftz) protein. Therefore, the homeodomain represents the DNA-binding domain of the homeotic proteins.

MeSH Terms
Animals Base Sequence Binding Sites DNA/metabolism DNA-Binding Proteins/genetics,isolation & purification,metabolism Genes, Homeobox Kinetics Molecular Sequence Data Protein Conformation Ultracentrifugation
Chemicals
DNA-Binding Proteins DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Müller M
Biozentrum der Universität Basel, Switzerland.
Affolter M
Leupin W
Otting G
Wüthrich K
Gehring W J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-12-20
Pages
4299-304
Language
English
Region
England
NLM ID
8208664
PMCID
PMC455145
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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