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PMID: 2905422 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Positional and developmental regulation of glutamine synthetase expression in mouse liver.

Molecular and cellular biology ·Vol. 8 ·No. 11 ·1988-11-00 ·Pages 4966-71

Kuo CF, Paulson KE, Darnell JE

Abstract

In situ hybridization showed that all fetal hepatocytes contain glutamine synthetase (GS) mRNA but that in adult mouse liver, only a single cell layer surrounding the central veins contains GS mRNA. A shift from the fetal to the adult pattern begins within a few days of birth and is complete within 12 days of birth. Since the total GS mRNA and the transcription rate of the single GS gene are similar at birth and in adults, we conclude that there is a generalized reduction in GS transcription for most hepatocytes and a sharp rise in GS transcription for the immediate pericentral cells. This may be a case of positional regulation of specific gene transcription in apparently a single cell lineage.

MeSH Terms
Animals Gene Expression Regulation Glutamate-Ammonia Ligase/genetics,metabolism Histocytochemistry Liver/enzymology,growth & development Mice RNA, Messenger/genetics,metabolism Transcription, Genetic
Chemicals
RNA, Messenger Glutamate-Ammonia Ligase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kuo C F
Rockefeller University, New York, New York 10021.
Paulson K E
Darnell J E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.11.4966-4971.1988
Published
1988-11-00
Pages
4966-71
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365590
Subset
IM
Grants
NCI NIH HHS · CA16006-14 · United States
NCI NIH HHS · CA18213-12 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.11.4966-4971.1988 PMC Full Text View journal details
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