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PMID: 6731793 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Quantitative electrophoretic transfer of DNA from polyacrylamide or agarose gels to nitrocellulose.

Analytical biochemistry ·Vol. 137 ·No. 1 ·1984-02-00 ·Pages 120-4

Smith MR, Devine CS, Cohn SM, Lieberman MW

Abstract

A method for efficient electrophoretic transfer of DNA fragments from polyacrylamide gels to nitrocellulose sheets was developed. Hybridization to these fragments can be performed by standard techniques. The method is also applicable to agarose gels, allowing this transfer method to be used for DNA ranging from 40 to at least 23,000 bp.

MeSH Terms
Autoradiography Collodion DNA/isolation & purification Electrophoresis, Polyacrylamide Gel/methods Gels Hydrogen-Ion Concentration Molecular Weight Nucleic Acid Hybridization
Chemicals
Gels Collodion DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Smith M R
Devine C S
Cohn S M
Lieberman M W
Article Info
Journal
Analytical biochemistry
Abbr.
Anal Biochem
ISSN
0003-2697
DOI
10.1016/0003-2697(84)90356-7
Published
1984-02-00
Pages
120-4
Language
English
Region
United States
NLM ID
0370535
Subset
IM
Grants
NCI NIH HHS · CA20513 · United States
NCI NIH HHS · CA31734 · United States
NIEHS NIH HHS · ES07066 · United States
External Links
PubMed source DOI: 10.1016/0003-2697(84)90356-7 View journal details
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