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PMID: 2903501 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Conserved upstream sequences of human class II major histocompatibility genes enhance expression of class II genes in wild-type but not mutant B-cell lines.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 21 ·1988-11-00 ·Pages 8186-90

Sloan JH, Boss JM

Abstract

Class II major histocompatibility genes contain a conserved upstream sequence (CUS) that is important in the expression of these genes. This region has been divided into two major elements, the X box and the Y box. The ability of these elements to mediate transcription of a heterologous promoter was assayed upon transfection into a B-cell line (Raji), a class II-specific trans-acting factor-deficient B-cell line (RJ2.2.5 cells), and a T-cell line (Jurkat). The results showed that the X box element was responsible for directing tissue-specific expression when Raji cells were compared to Jurkat cells. The X box could not direct expression of the heterologous promoter in the trans-acting factor-deficient cell line, indicating that the X box is an ultimate target of the missing or defective factor in the RJ2.2.5 cell line. The Y box directed an equal but extremely low level of transcription in this system in both the mutant and wild-type B-cell lines, suggesting that this element is not involved in B-cell expression or as a target of the mutant factor.

MeSH Terms
B-Lymphocytes/metabolism Base Sequence Cell Line Gene Expression Regulation Genes, Homeobox Genes, MHC Class II Humans Mutation Transcription, Genetic Transfection
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sloan J H
Department of Microbiology and Immunology, Emory University, Atlanta, GA.
Boss J M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.21.8186
Published
1988-11-00
Pages
8186-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282392
Subset
IM
Grants
NCRR NIH HHS · S07 RR05264 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.21.8186 PMC Full Text View journal details
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