Home LiteratureArticle Details
PMID: 3317416 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Defective HLA class II expression in a regulatory mutant is partially complemented by activated ras oncogenes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 23 ·1987-12-00 ·Pages 8603-7

Hume CR, Accolla RS, Lee JS

Abstract

The human B-cell line RJ2.2.5, derived by mutagenesis from a Burkitt lymphoma cell line and selected for loss of HLA class II antigen expression, was infected with recombinant retroviruses containing either the Harvey murine sarcoma virus oncogene v-Ha-ras or the human neuroblastoma homolog NRAS. Both activated ras genes partially complemented the regulatory defect in RJ2.2.5 and specifically increased the expression of the DR and DQ subsets of HLA class II genes. Blot-hybridization analysis and RNase mapping indicated that HLA-DQ alpha-chain mRNA in the infected cell lines was increased to a level at least 50% that of the parent B-cell line, Raji. The levels of HLA-DR and -DQ beta-chain RNA also were increased but to a lesser extent. In contrast, we detected no effect of ras on the quantities of other class II, class I, or invariant-chain mRNAs. Fluorescence-activated cell sorter analysis with antibodies recognizing HLA-DR, -DQ, and class I antigens supported these observations. Enhancement of HLA class II gene expression by ras genes may have important implications for regulation of the immune system in response to transformation.

MeSH Terms
Antigens, Surface/genetics Cell Line Flow Cytometry Gene Expression Regulation Genes, ras HLA-D Antigens/genetics Humans Oncogene Proteins, Viral/physiology RNA, Messenger/genetics Transcription, Genetic
Chemicals
Antigens, Surface HLA-D Antigens Oncogene Proteins, Viral RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hume C R
Immunology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Accolla R S
Lee J S
References (49)
49 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. A rapid and improved method for generating cDNA libraries in plasmid and phage lambda vectors.
    Gene. 1987;56(2-3):301-7 PMID: 2445632
  3. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 PMID: 286319
  4. The human Ia system.
    Adv Immunol. 1979;28:221-92 PMID: 94505
  5. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
    Methods Enzymol. 1980;65(1):718-49 PMID: 6154876
  6. Two populations of Ia-like molecules on a human B cell line.
    J Immunol. 1980 Jul;125(1):293-9 PMID: 6966655
  7. Identification of a trans-acting function regulation HLA-DR expression in a DR-negative B cell variant.
    Somatic Cell Genet. 1980 Mar;6(2):285-98 PMID: 6967232
  8. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):616-20 PMID: 6165999
  9. The p21 src genes of Harvey and Kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes.
    Nature. 1981 Aug 6;292(5823):506-11 PMID: 6265801
  10. Increased expression of I-region-associated antigen (Ia) on B cells after cross-linking of surface immunoglobulin.
    J Immunol. 1981 Sep;127(3):881-8 PMID: 6790620
  11. Sequence of an HLA-DR alpha-chain cDNA clone and intron-exon organization of the corresponding gene.
    Nature. 1982 Oct 21;299(5885):750-2 PMID: 6811954
  12. Enhanced expression of human Ia antigens by chronic lymphocytic leukemia cells following treatment with 12-O-tetradecanoylphorbol-13-acetate.
    Eur J Immunol. 1983 Feb;13(2):156-9 PMID: 6403357
  13. Human B cell variants immunoselected against a single Ia antigen subset have lost expression of several Ia antigen subsets.
    J Exp Med. 1983 Mar 1;157(3):1053-8 PMID: 6403646
  14. Isolation of cDNA clones for the p33 invariant chain associated with HLA-DR antigens.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5714-8 PMID: 6412232
  15. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  16. Expression of class I major histocompatibility antigens switched off by highly oncogenic adenovirus 12 in transformed rat cells.
    Nature. 1983 Oct 27-Nov 2;305(5937):771-5 PMID: 6355856
  17. Identification by transfection of transforming sequences in DNA of human T-cell leukemias.
    Proc Natl Acad Sci U S A. 1983 Nov;80(21):6676-9 PMID: 6605534
  18. A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes.
    Immunogenetics. 1984;19(3):179-94 PMID: 6200433
  19. Ia-negative B-cell variants reveal a coordinate regulation in the transcription of the HLA class II gene family.
    Immunogenetics. 1984;19(4):349-53 PMID: 6609124
  20. Surface antigens of melanoma and melanocytes. Specificity of induction of Ia antigens by human gamma-interferon.
    J Exp Med. 1984 Jul 1;160(1):255-69 PMID: 6204001
  21. Construction and applications of a highly transmissible murine retrovirus shuttle vector.
    Cell. 1984 Jul;37(3):1053-62 PMID: 6331674
  22. B cell origin of non-T cell acute lymphoblastic leukemia. A model for discrete stages of neoplastic and normal pre-B cell differentiation.
    J Clin Invest. 1984 Aug;74(2):332-40 PMID: 6378973
  23. Characterization and expression of the HLA-DC antigens defined by anti-Leu 10.
    Hum Immunol. 1984 Aug;10(4):221-35 PMID: 6332101
  24. Interleukin-induced increase in Ia expression by normal mouse B cells.
    J Exp Med. 1984 Sep 1;160(3):679-94 PMID: 6432933
  25. High-efficiency gene transfer into mammalian cells: generation of helper-free recombinant retrovirus with broad mammalian host range.
    Proc Natl Acad Sci U S A. 1984 Oct;81(20):6349-53 PMID: 6093098
  26. Transcriptional regulation of HLA class II and invariant chain genes.
    J Immunol. 1985 Jan;134(1):637-40 PMID: 3964823
  27. Antigen-specific interaction between T and B cells.
    Nature. 1985 Apr 11-17;314(6011):537-9 PMID: 3157869
  28. A defect in the regulation of major histocompatibility complex class II gene expression in human HLA-DR negative lymphocytes from patients with combined immunodeficiency syndrome.
    J Clin Invest. 1985 Jul;76(1):381-5 PMID: 3860509
  29. Reactivation by a trans-acting factor of human major histocompatibility complex Ia gene expression in interspecies hybrids between an Ia-negative human B-cell variant and an Ia-positive mouse B-cell lymphoma.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):5145-9 PMID: 3875096
  30. Genetic complexity and expression of human class II histocompatibility antigens.
    Immunol Rev. 1985 Jul;85:45-86 PMID: 3899915
  31. Trans-acting element(s) operating across species barriers positively regulate expression of major histocompatibility complex class II genes.
    J Exp Med. 1985 Oct 1;162(4):1117-33 PMID: 3862745
  32. Rapid transfer of DNA from agarose gels to nylon membranes.
    Nucleic Acids Res. 1985 Oct 25;13(20):7207-21 PMID: 4059056
  33. DO beta: a new beta chain gene in HLA-D with a distinct regulation of expression.
    EMBO J. 1985 Nov;4(11):2839-47 PMID: 2998758
  34. A trans-acting class II regulatory gene unlinked to the MHC controls expression of HLA class II genes.
    Nature. 1985 Nov 21-27;318(6043):291-3 PMID: 3934559
  35. Influence of allelic polymorphism on the assembly and surface expression of class II MHC (Ia) molecules.
    Cell. 1985 Nov;43(1):233-42 PMID: 3935317
  36. ras-transformed cells: altered levels of phosphatidylinositol-4,5-bisphosphate and catabolites.
    Science. 1986 Jan 24;231(4736):407-10 PMID: 3001936
  37. aIr-1, a newly found locus on mouse chromosome 16 encoding a trans-acting activator factor for MHC class II gene expression.
    J Exp Med. 1986 Jul 1;164(1):369-74 PMID: 3088202
  38. Normal p21N-ras couples bombesin and other growth factor receptors to inositol phosphate production.
    Nature. 1986 Sep 11-17;323(6084):173-6 PMID: 3018591
  39. Stable integration of mouse DNA into Ia-negative human B-lymphoma cells causes reexpression of the human Ia-positive phenotype.
    Proc Natl Acad Sci U S A. 1986 Oct;83(19):7415-8 PMID: 3489937
  40. Class II histocompatibility antigen expression in human melanocytes transformed by Harvey murine sarcoma virus (Ha-MSV) and Kirsten MSV retroviruses.
    J Exp Med. 1986 Nov 1;164(5):1710-22 PMID: 2430046
  41. N-myc amplification causes down-modulation of MHC class I antigen expression in neuroblastoma.
    Cell. 1986 Dec 5;47(5):667-74 PMID: 3096575
  42. Interferon-gamma suppresses B cell stimulation factor (BSF-1) induction of class II MHC determinants on B cells.
    J Immunol. 1986 Dec 1;137(11):3534-7 PMID: 3097125
  43. BSF1 induces membrane protein phosphorylation but not phosphoinositide metabolism, Ca2+ mobilization, protein kinase C translocation, or membrane depolarization in resting murine B lymphocytes.
    J Immunol. 1986 Dec 1;137(11):3664-70 PMID: 3023486
  44. Synergism of v-myc and v-Ha-ras in the in vitro neoplastic progression of murine lymphoid cells.
    Mol Cell Biol. 1986 Sep;6(9):3221-31 PMID: 3023969
  45. Elevated levels of glucose transport and transporter messenger RNA are induced by ras or src oncogenes.
    Science. 1987 Mar 20;235(4795):1492-5 PMID: 3103217
  46. Expression of human class II major histocompatibility complex antigens using retrovirus vectors.
    Proc Natl Acad Sci U S A. 1987 Apr;84(8):2150-4 PMID: 3031667
  47. Biological effects of a murine retrovirus carrying an activated N-ras gene of human origin.
    Virology. 1987 May;158(1):69-78 PMID: 3576974
  48. Methylation of the HLA-DR alpha gene is positively correlated with expression.
    Immunogenetics. 1987;26(1-2):92-8 PMID: 2440802
  49. Stable variants affecting B cell alloantigens in human lymphoid cells.
    Nature. 1978 Feb 2;271(5644):459-61 PMID: 75508
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.23.8603
Published
1987-12-00
Pages
8603-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299593
Subset
IM
Grants
NCI NIH HHS · 2P01 CA20194-10 · United States
NCI NIH HHS · CA-08748 · United States
NCRR NIH HHS · RR-05534 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.23.8603 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2