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PMID: 2898471 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Pilin-gene phase variation of Moraxella bovis is caused by an inversion of the pilin genes.

Journal of bacteriology ·Vol. 170 ·No. 7 ·1988-07-00 ·Pages 3032-9

Marrs CF, Ruehl WW, Schoolnik GK, Falkow S

Abstract

Moraxella bovis Epp63 can express either of two different pilin proteins, called alpha and beta. We have previously cloned and sequenced the beta-pilin gene and now report that DNAs isolated from bacteria expressing alpha pilin have hybridization patterns consistently different from those of bacteria expressing beta pilin. The phase variation between alpha- and beta-pilin gene expression appears to be associated with an inversion of about 2 kilobases of DNA, whose endpoints occur within the coding region of the expressed pilin gene. Comparisons of the beta-pilin gene sequence with those of well-studied bacterial inversion systems revealed a stretch of 58% sequence similarity (21 of 36 base pairs) between the left inverted repeat of the Salmonella typhimurium flagellar hin control region and the amino-terminal portion of the beta-pilin gene.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics Base Sequence Chromosome Inversion DNA Restriction Enzymes DNA, Bacterial/genetics Electrophoresis, Polyacrylamide Gel Fimbriae Proteins Fimbriae, Bacterial Gene Expression Regulation Genes, Bacterial Immunoassay Molecular Sequence Data Moraxella/genetics Nucleic Acid Hybridization Sequence Homology, Nucleic Acid
Chemicals
Bacterial Outer Membrane Proteins DNA, Bacterial Fimbriae Proteins DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Marrs C F
Department of Epidemiology, University of Michigan, Ann Arbor 48109.
Ruehl W W
Schoolnik G K
Falkow S
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.7.3032-3039.1988
Published
1988-07-00
Pages
3032-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211245
Subset
IM
Grants
NIAID NIH HHS · AI 10885 · United States
NEI NIH HHS · BM-1 1R01EY07125-01 · United States
External Links
PubMed source DOI: 10.1128/jb.170.7.3032-3039.1988 PMC Full Text View journal details
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