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PMID: 6273384 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Homology between the invertible deoxyribonucleic acid sequence that controls flagellar-phase variation in Salmonella sp. and deoxyribonucleic acid sequences in other organisms.

Journal of bacteriology ·Vol. 148 ·No. 3 ·1981-12-00 ·Pages 829-36

Szekely E, Simon M

Abstract

The invertible deoxyribonucleic acid (DNA) segment cloned from Salmonella sp. was radioactively labeled and used as a probe to search for homologous sequences by Southern hybridization. Only one copy of the invertible segment could be found on the Salmonella sp. genome. Partial sequence homology with the invertible region was detected in bacteriophage Mu and P1 DNA by low-stringency hybridization. Under these conditions, no homology was detected with Escherichia coli DNA. A strain of Salmonella sp. defective in phase variation carrying the vH2- allele was also analyzed by DNA-DNA hybridization. The results show that there is sequence divergence between diphasic and vH2- strains within the invertible sequence.

MeSH Terms
Bacterial Proteins/genetics Base Sequence Coliphages/genetics DNA Restriction Enzymes DNA, Bacterial Deoxyribonucleases, Type II Site-Specific Escherichia coli/genetics Flagellin/genetics Genes, Bacterial Nucleic Acid Hybridization Salmonella/genetics
Chemicals
Bacterial Proteins DNA, Bacterial Flagellin DNA Restriction Enzymes endodeoxyribonuclease HpaI Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Szekely E
Simon M
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23 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.148.3.829-836.1981
Published
1981-12-00
Pages
829-36
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216281
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.148.3.829-836.1981 PMC Full Text View journal details
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