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PMID: 2877439 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Sequence and transcription analysis of the Petunia mitochondrial gene for the ATP synthase proteolipid subunit.

Nucleic acids research ·Vol. 14 ·No. 20 ·1986-10-24 ·Pages 7995-8006

Young EG, Hanson MR, Dierks PM

Abstract

We have sequenced the Petunia hybrida gene that specifies the proteolipid subunit of the mitochondrial Fo ATP synthase and have used this gene to investigate plant mitochondrial gene transcription. The Petunia atp 9 gene contains a single open-reading frame capable of specifying a 77 amino acid-polypeptide that is homologous to bovine, fungal and maize proteolipid subunits. S1 protection identified 3 transcripts in a ratio of 1:5:100 in the Petunia tissues tested. The transcripts share a common 3' terminus but have 5' termini that map 528, 266, and 121 nucleotides upstream of the translation start site. The 5' terminus of the longest transcript maps to the sequence ATATAGTA, which is nearly identical to the yeast mitochondrial transcription initiation site ATATAAGTA. Primer extension analysis indicates that these two shorter transcripts are not due to splicing. The two shorter transcripts originate at sequences homologous to sites at 5' termini of two pea and maize genes. These consensus sequences may signal processing events other than splicing.

MeSH Terms

Amino Acid Sequence Base Sequence Chromosome Mapping DNA, Mitochondrial/genetics Genes Macromolecular Substances Plants/genetics Proteolipids/genetics Proton-Translocating ATPases/genetics RNA Processing, Post-Transcriptional Sequence Homology, Nucleic Acid Transcription, Genetic

Chemicals

DNA, Mitochondrial Macromolecular Substances Proteolipids Proton-Translocating ATPases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Young E G

Hanson M R

Dierks P M

References (22)

22 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/14.20.7995

Published

1986-10-24

Pages

7995-8006

Language

English

Region

England

NLM ID

0411011

PMCID

PMC311830

Subset

Databases

GENBANK

X04504

External Links

PubMed source DOI: 10.1093/nar/14.20.7995 PMC Full Text View journal details

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Sequence and transcription analysis of the Petunia mitochondrial gene for the ATP synthase proteolipid subunit.

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