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PMID: 3856873 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of a yeast mitochondrial promoter by deletion mutagenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 7 ·1985-04-00 ·Pages 1954-8

Biswas TK, Edwards JC, Rabinowitz M, Getz GS

Abstract

We have generated collections of mutants of the promoter for the small rRNA gene from the mitochondria of yeast deleted from either the 3' or 5' end. Plasmids containing the partially deleted promoter were assayed for their ability to direct correct transcriptional initiation in a homologous in vitro system. We find that the region required for high-efficiency promoter function lies between positions -10 and +2. Our methods detected no effect of flanking sequences on the strength of this promoter.

MeSH Terms
Base Sequence Chromosome Deletion Mitochondria/ultrastructure Mutation Plasmids Promoter Regions, Genetic Transcription, Genetic Yeasts/genetics
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Biswas T K
Edwards J C
Rabinowitz M
Getz G S
References (22)
22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.7.1954
Published
1985-04-00
Pages
1954-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397457
Subset
IM
Grants
NIGMS NIH HHS · 5T32GM07281 · United States
NHLBI NIH HHS · HL 04442 · United States
Databases
GENBANK
M11102
External Links
PubMed source DOI: 10.1073/pnas.82.7.1954 PMC Full Text View journal details
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