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PMID: 2874560 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Tyrosine 3-hydroxylase in rat brain and adrenal medulla: hybridization histochemistry and immunohistochemistry combined with retrograde tracing.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 16 ·1986-08-00 ·Pages 6208-12

Schalling M, Hökfelt T, Wallace B, Goldstein M, Filer D, Yamin C, Schlesinger DH

Abstract

Rat brain and adrenal gland were analyzed by hybridization histochemistry using an RNA probe complementary to mRNA for tyrosine 3-hydroxylase (TyrOHase; tyrosine 3-monooxygenase, EC 1.14.16.2), by immunohistochemistry using TyrOHase antiserum, and by retrograde tracing using the fluorescent compound Fast blue. Cell bodies in the ventral mesencephalon contained mRNA for TyrOHase, and these cells were also TyrOHase immunoreactive. After injection of Fast blue into the striatum, such double-labeled cells in addition contained the retrograde tracer, showing that these cells send axonal projections to the injection site. These results show that hybridization histochemistry can be used to identify transmitter-specific neuron populations and that their projections can be established.

MeSH Terms
Adrenal Medulla/cytology,enzymology Animals Autoradiography Brain/cytology,enzymology Fluorescent Antibody Technique Histocytochemistry Male Mesencephalon/enzymology Microscopy, Fluorescence Nucleic Acid Hybridization RNA, Messenger/analysis,genetics Rats Rats, Inbred Strains Sulfur Radioisotopes Tissue Distribution Tyrosine 3-Monooxygenase/genetics
Chemicals
RNA, Messenger Sulfur Radioisotopes Tyrosine 3-Monooxygenase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Schalling M
Hökfelt T
Wallace B
Goldstein M
Filer D
Yamin C
Schlesinger D H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.16.6208
Published
1986-08-00
Pages
6208-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386469
Subset
IM
Grants
PHS HHS · 02717-28 · United States
PHS HHS · 06801-19 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.16.6208 PMC Full Text View journal details
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