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PMID: 2863748 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Accurate and efficient transcription of human c-myc genes injected into Xenopus laevis oocytes.

Molecular and cellular biology ·Vol. 5 ·No. 6 ·1985-06-00 ·Pages 1434-41

Nishikura K, Goldflam S, Vuocolo GA

Abstract

We investigated the expression of the cloned human c-myc gene in Xenopus laevis oocytes microinjected with different recombinants. We found that microinjected plasmid DNA carrying an intact human c-myc gene directs efficient and faithful transcription from its own two promoters in X. laevis oocytes. This active transcription was unaffected by the presence of previously identified enhancing elements such as simian virus 72-base pair repeats or mouse immunoglobulin heavy-chain gene enhancer sequences in the construct in cis. This suggests that all necessary DNA sequences for accurate and faithful transcription recognized by the transcription machinery of the frog oocyte are self-contained. In addition, we have found that human c-myc transcripts synthesized in oocytes are properly polyadenylated at either one of two sites and also that the transcripts are spliced correctly but with low efficiency.

MeSH Terms
Animals Cloning, Molecular Enhancer Elements, Genetic Female Humans Microinjections Oncogenes Oocytes/metabolism Poly A/metabolism Promoter Regions, Genetic RNA Processing, Post-Transcriptional RNA Splicing RNA, Messenger/metabolism Transcription, Genetic Xenopus laevis
Chemicals
RNA, Messenger Poly A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nishikura K
Goldflam S
Vuocolo G A
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64 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.6.1434-1441.1985
Published
1985-06-00
Pages
1434-41
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366874
Subset
IM
Grants
NIGMS NIH HHS · GM-31060 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.6.1434-1441.1985 PMC Full Text View journal details
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