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PMID: 6327285 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Synthesis of two mRNAs by utilization of alternate polyadenylation sites: expression of SV40-mouse immunoglobulin mu chain gene recombinants in Cos monkey cells.

The EMBO journal ·Vol. 3 ·No. 4 ·1984-04-00 ·Pages 689-99

Nishikura K, Vuocolo GA

Abstract

The immunoglobulin mu gene encodes both membrane-bound (m) and secreted (s) mu chains. Two mRNAs, mum and mus, which differ only at their 3' ends and encode the two mu polypeptides, are produced differentially during maturation of B cells (bearing membrane-bound IgM) to plasma cells (secreting IgM). We have constructed a simian virus 40 (SV40)-mouse immunoglobulin mu chain chimeric recombinant carrying the SV40 early promoter and a part of the constant region of the mu gene, and also various deletion mutants of the recombinant. When the recombinant is introduced into Cos monkey cells, both mum and mus type mRNAs are synthesized. These two messages are polyadenylated at the mum and mus poly(A) addition sites, respectively. Correct splicing of the mu transcripts is also observed with reasonable efficiency. Analysis of the transcripts from the deletion mutants lacking either the mum or mus poly(A) addition site has indicated that the selection of the polyadenylation sites is determined simply by the proximity of the site to the promoter. This suggests that the machinery for 3' end formation of the messages, which probably determine the two alternate RNA processing pathways for mus and mum mRNAs, may scan in a promotor proximal to distal direction.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Haplorhini Immunoglobulin Heavy Chains/genetics Immunoglobulin mu-Chains/genetics Mice Mice, Inbred BALB C Mutation Operon Poly A/metabolism RNA, Messenger/biosynthesis Simian virus 40/genetics Transcription, Genetic
Chemicals
DNA, Recombinant Immunoglobulin Heavy Chains Immunoglobulin mu-Chains RNA, Messenger Poly A DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nishikura K
Vuocolo G A
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64 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-04-00
Pages
689-99
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557413
Subset
IM
Grants
NIGMS NIH HHS · GM-31060 · United States
External Links
PubMed source PMC Full Text View journal details
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