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PMID: 2847916 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A physical map of the human pseudoautosomal region.

The EMBO journal ·Vol. 7 ·No. 8 ·1988-08-00 ·Pages 2377-85

Brown WR

Abstract

A physical map of the human pseudoautosomal region has been constructed using pulsed field gel electrophoresis and the infrequently cutting restriction enzymes BssHIII, EagI, SstII, NotI, MluI and NruI. This map extends 2.3 Mbp from the telomere to sex-chromosome-specific DNA, includes at least seven CpG islands and locates four genetically mapped loci. Five of the CpG islands are organized into two clusters. One cluster is adjacent to the telomere, the other extends into sex-chromosome-specific DNA. There is congruence between the genetic and physical maps which implies that the frequency of recombination is approximately uniform throughout the DNA.

MeSH Terms

Cell Line Chromosome Mapping DNA/genetics DNA Restriction Enzymes Electrophoresis, Agar Gel Female Humans Hybrid Cells Male Multigene Family Nucleic Acid Hybridization Sex Chromosomes Turner Syndrome/genetics

Chemicals

DNA DNA Restriction Enzymes

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Brown W R

Biochemistry Department, Oxford University, UK.

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1988-08-00

Pages

2377-85

Language

English

Region

England

NLM ID

8208664

PMCID

PMC457103

Subset

External Links

PubMed source PMC Full Text View journal details

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A physical map of the human pseudoautosomal region.

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