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PMID: 2845860 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples.

Applied and environmental microbiology ·Vol. 54 ·No. 8 ·1988-08-00 ·Pages 1983-8

Lewis GD, Metcalf TG

Abstract

Polyethylene glycol 6000 precipitation was found to be an effective concentration method that enhanced the chances for detecting human virus pathogens in environmental samples. Percent recoveries from eluates of fresh and estuarine waters with 8% polyethylene glycol 6000 averaged 86 for hepatitis A virus, 77 for human rotavirus Wa, 87 for simian rotavirus SA11, and 68 for poliovirus. Percent recoveries of 97, 40, 97 and 105, respectively, for the same viruses were obtained from oyster eluates by the same procedure. Percent recoveries of 97 for hepatitis A virus and 78 for human rotavirus Wa were obtained from sediment eluates containing 2 M NaNO3 with a final concentration of 15% polyethylene glycol 6000. The polyethylene glycol method was shown to be more effective than the organic flocculation method for recovery of hepatitis A virus and rotaviruses Wa and SA11, but not of poliovirus 1 in laboratory studies. In field trials, hepatitis A virus or rotavirus or both were recovered from 12 of 18 eluates by polyethylene glycol, compared with recovery from 9 of 18 eluates by organic flocculation from fresh and estuarine waters subject to pollution.

MeSH Terms
Animals Cell Line Chemical Precipitation Fresh Water Hepatovirus/isolation & purification Humans Ostreidae/microbiology Poliovirus/isolation & purification Polyethylene Glycols Rotavirus/isolation & purification Water Microbiology
Chemicals
Polyethylene Glycols
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lewis G D
Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77030.
Metcalf T G
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.54.8.1983-1988.1988
Published
1988-08-00
Pages
1983-8
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC202790
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.54.8.1983-1988.1988 PMC Full Text View journal details
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