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PMID: 6297395 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Simple apparatus for collecting estuarine sediments and suspended solids to detect solids-associated virus.

Applied and environmental microbiology ·Vol. 45 ·No. 1 ·1983-01-00 ·Pages 323-7

Metcalf TG, Melnick JL

Abstract

Laboratory trials of a new sampler for collection of estuarine sediment-associated virus resulted in a recovery effectiveness averaging 30% for two enteroviruses and rotavirus SA11. A minimal recovery potential of 54% was calculated when losses caused by virus concentration procedure inadequacies were excluded. Both sediment-associated and suspended solids-associated viruses were collected with the sampler. Recoveries of 61 and 60% poliovirus and rotavirus, respectively, were obtained from salt water-suspended, solids-associated virus. The unique advantage of the sampler for selective collection of virus-associated top layers of sediment, plus collection over extensive areas, resulted in recovery of more virus than was obtained with a commonly used dredge-type sampler.

MeSH Terms
Enterovirus/isolation & purification Microbiological Techniques/instrumentation Poliovirus/isolation & purification Rotavirus/isolation & purification Soil Microbiology Viruses/isolation & purification Water Microbiology
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Metcalf T G
Melnick J L
References (11)
11 references, click to expand
  1. An outbreak of hepatitis-A associated with recreational lake water.
    Am J Epidemiol. 1974 Feb;99(2):145-54 PMID: 4810629
  2. Oyster-associated hepatitis. Failure of shellfish certification programs to prevent outbreaks.
    JAMA. 1975 Sep 8;233(10):1065-8 PMID: 1174151
  3. Association of enteroviruses with natural and artificially introduced colloidal solids in water and infectivity of solids-associated virions.
    Appl Microbiol. 1975 Aug;30(2):212-22 PMID: 240308
  4. Detection of animal viruses in coastal seawater and sediments.
    Appl Microbiol. 1975 Sep;30(3):472-5 PMID: 170859
  5. Demonstration of solids-associated virus in wastewater and sludge.
    Appl Environ Microbiol. 1976 Mar;31(3):354-8 PMID: 180882
  6. Development of a quantitative method for detecting enteroviruses in estuarine sediments.
    Appl Environ Microbiol. 1977 Aug;34(2):158-63 PMID: 20839
  7. Role of sediment in the persistence of enteroviruses in the estuarine environment.
    Appl Environ Microbiol. 1978 Apr;35(4):685-9 PMID: 206204
  8. A plaque assay for the simian rotavirus SAII.
    J Gen Virol. 1979 Jun;43(3):513-9 PMID: 225432
  9. An Australia-wide outbreak of gastroenteritis from oysters caused by Norwalk virus.
    Med J Aust. 1979 Oct 6;2(7):329-33 PMID: 514174
  10. Norwalk gastrointestinal illness: an outbreak associated with swimming in a recreational lake and secondary person-to-person transmission.
    Am J Epidemiol. 1982 Feb;115(2):163-72 PMID: 6277184
  11. [Hepatitis epidemic transmitted by oysters].
    Sven Lakartidn. 1956 Apr 20;53(16):989-1003 PMID: 13324708
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
Published
1983-01-00
Pages
323-7
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC242279
Subset
IM
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