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PMID: 3038016 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

An A-ELISA to detect hepatitis A virus in estuarine samples.

Applied and environmental microbiology ·Vol. 53 ·No. 5 ·1987-05-00 ·Pages 1192-5

Nasser AM, Metcalf TG

Abstract

An amplified enzyme-linked immunosorbent assay (A-ELISA) for detecting and quantifying hepatitis A virus in estuarine water samples is described. The test was five times more sensitive than a standard ELISA and at least two times more sensitive than radioimmunoassay. Test sensitivity was unaffected by the procedures used to concentrate the virus in estuarine samples or by the presence of humic and tannic acids in test samples. Nonspecific reactions were not encountered with a number of enteroviruses or with a rotavirus. A high sensitivity and specificity combined with speed, low cost, and freedom from radiolabels made the A-ELISA useful for detecting hepatitis A virus in environmental samples. The virus was detected in 3 of 20 estuarine water samples examined by A-ELISA.

MeSH Terms
Animals Cell Line Enzyme-Linked Immunosorbent Assay Hepatovirus/isolation & purification Predictive Value of Tests Radioimmunoassay Water Microbiology
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nasser A M
Metcalf T G
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16 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.53.5.1192-1195.1987
Published
1987-05-00
Pages
1192-5
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC203834
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.53.5.1192-1195.1987 PMC Full Text View journal details
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