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PMID: 2845398 Published · ppublish English Journal Article

Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 19 ·1988-10-00 ·Pages 7159-63

Cotecchia S, Schwinn DA, Randall RR, Lefkowitz RJ, Caron MG, Kobilka BK

Abstract

The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the receptor protein purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue polypeptide that shows the most sequence identity with the other adrenergic receptors and the putative protein product of the related clone G-21. Similarities with the muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm that we have cloned the alpha 1-adrenergic receptor that couples to inositol phospholipid metabolism.

MeSH Terms
Animals Base Sequence Cloning, Molecular Cricetinae DNA/analysis GTP-Binding Proteins/metabolism Gene Expression Regulation Molecular Sequence Data Phosphatidylinositols/metabolism Receptors, Adrenergic, alpha/genetics
Chemicals
Phosphatidylinositols Receptors, Adrenergic, alpha DNA GTP-Binding Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cotecchia S
Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Schwinn D A
Randall R R
Lefkowitz R J
Caron M G
Kobilka B K
References (33)
33 references, click to expand
  1. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.
    Biochem J. 1973 Jul;133(3):529-39 PMID: 4733239
  2. Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype.
    Proc Natl Acad Sci U S A. 1988 Sep;85(17):6301-5 PMID: 2842764
  3. Molecular mechanisms involved in alpha-adrenergic responses.
    Mol Cell Endocrinol. 1981 Sep;23(3):233-64 PMID: 6269923
  4. Characterization of the alpha 1-adrenergic receptor subtype in a smooth muscle cell line.
    J Biol Chem. 1982 Jan 25;257(2):694-7 PMID: 6119313
  5. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  6. Validation and statistical analysis of a computer modeling method for quantitative analysis of radioligand binding data for mixtures of pharmacological receptor subtypes.
    Mol Pharmacol. 1982 Jan;21(1):5-16 PMID: 6982395
  7. Photoaffinity labeling of mammalian alpha 1-adrenergic receptors. Identification of the ligand binding subunit with a high affinity radioiodinated probe.
    J Biol Chem. 1984 Feb 25;259(4):2579-87 PMID: 6321475
  8. Inositol trisphosphate and diacylglycerol as second messengers.
    Biochem J. 1984 Jun 1;220(2):345-60 PMID: 6146314
  9. Catecholamines are mitogenic in 3T3 and bovine aortic endothelial cells.
    J Clin Invest. 1984 Aug;74(2):483-7 PMID: 6611353
  10. Induction of DNA synthesis in cultured rat hepatocytes through stimulation of alpha 1 adrenoreceptor by norepinephrine.
    Science. 1985 Feb 15;227(4688):749-51 PMID: 2982212
  11. Synthetic oligonucleotide probes deduced from amino acid sequence data. Theoretical and practical considerations.
    J Mol Biol. 1985 May 5;183(1):1-12 PMID: 4009718
  12. Adrenergic regulation of gluconeogenesis: possible involvement of two mechanisms of signal transduction in alpha 1-adrenergic action.
    Proc Natl Acad Sci U S A. 1985 Oct;82(20):6727-30 PMID: 2995981
  13. Phorbol ester effects on alpha 1-adrenoceptor binding and phosphatidylinositol metabolism in cultured vascular smooth muscle cells.
    Life Sci. 1985 Dec 23;37(25):2389-98 PMID: 3001461
  14. Characterization of alpha 1-adrenergic receptor subtypes in rat brain: a reevaluation of [3H]WB4104 and [3H]prazosin binding.
    Mol Pharmacol. 1986 Apr;29(4):321-30 PMID: 3010073
  15. Cloning of the gene and cDNA for mammalian beta-adrenergic receptor and homology with rhodopsin.
    Nature. 1986 May 1-7;321(6065):75-9 PMID: 3010132
  16. Mammalian alpha 1-adrenergic receptor. Purification and characterization of the native receptor ligand binding subunit.
    J Biol Chem. 1986 Jun 15;261(17):7710-6 PMID: 3011796
  17. Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor.
    Nature. 1986 Oct 2-8;323(6087):411-6 PMID: 3762692
  18. Primary structure of porcine cardiac muscarinic acetylcholine receptor deduced from the cDNA sequence.
    FEBS Lett. 1986 Dec 15;209(2):367-72 PMID: 3792556
  19. cDNA for the human beta 2-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor.
    Proc Natl Acad Sci U S A. 1987 Jan;84(1):46-50 PMID: 3025863
  20. Regulation of adrenergic receptor function by phosphorylation. I. Agonist-promoted desensitization and phosphorylation of alpha 1-adrenergic receptors coupled to inositol phospholipid metabolism in DDT1 MF-2 smooth muscle cells.
    J Biol Chem. 1987 Mar 5;262(7):3098-105 PMID: 3029100
  21. Regulation of adrenergic receptor function by phosphorylation. II. Effects of agonist occupancy on phosphorylation of alpha 1- and beta 2-adrenergic receptors by protein kinase C and the cyclic AMP-dependent protein kinase.
    J Biol Chem. 1987 Mar 5;262(7):3106-13 PMID: 3029101
  22. Differentiation of alpha 1-adrenergic receptors linked to phosphatidylinositol turnover and cyclic AMP accumulation in rat brain.
    Mol Pharmacol. 1987 Mar;31(3):239-46 PMID: 2436033
  23. Primary structure and biochemical properties of an M2 muscarinic receptor.
    Science. 1987 May 1;236(4801):600-5 PMID: 3107123
  24. A family of receptors coupled to guanine nucleotide regulatory proteins.
    Biochemistry. 1987 May 19;26(10):2657-64 PMID: 3038163
  25. Molecular biology of the visual pigments.
    Vision Res. 1986;26(12):1881-95 PMID: 3303660
  26. G proteins: transducers of receptor-generated signals.
    Annu Rev Biochem. 1987;56:615-49 PMID: 3113327
  27. An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins.
    Nature. 1987 Sep 3-9;329(6134):75-9 PMID: 3041227
  28. Alpha 1-adrenoceptor subtypes linked to different mechanisms for increasing intracellular Ca2+ in smooth muscle.
    Nature. 1987 Sep 24-30;329(6137):333-5 PMID: 2442626
  29. Use of eukaryotic expression technology in the functional analysis of cloned genes.
    Methods Enzymol. 1987;152:684-704 PMID: 3657593
  30. Cloning, sequencing, and expression of the gene coding for the human platelet alpha 2-adrenergic receptor.
    Science. 1987 Oct 30;238(4827):650-6 PMID: 2823383
  31. Glycosylation of the mammalian alpha 1-adrenergic receptor by complex type N-linked oligosaccharides.
    Mol Pharmacol. 1987 Nov;32(5):565-71 PMID: 2824978
  32. Cloning of the cDNA for the human beta 1-adrenergic receptor.
    Proc Natl Acad Sci U S A. 1987 Nov;84(22):7920-4 PMID: 2825170
  33. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.19.7159
Published
1988-10-00
Pages
7159-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282143
Subset
IM
Databases
GENBANK
J04084
External Links
PubMed source DOI: 10.1073/pnas.85.19.7159 PMC Full Text View journal details
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