Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 17 ·1988-09-00 ·Pages 6301-5

Regan JW, Kobilka TS, Yang-Feng TL, Caron MG, Lefkowitz RJ, Kobilka BK

Abstract

An alpha 2-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet alpha 2-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet alpha 2-adrenergic receptor and is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the alpha 2-adrenergic ligand [3H]rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the alpha 2B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet alpha 2-adrenergic receptor (alpha 2A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective alpha-adrenergic ligands.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cell Line Chromosomes, Human, Pair 4 Cloning, Molecular DNA/genetics Genes Humans Kidney/metabolism Kinetics Models, Molecular Molecular Sequence Data Protein Conformation Receptors, Adrenergic, alpha/genetics,metabolism Transcription, Genetic

Chemicals

Receptors, Adrenergic, alpha DNA

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Regan J W

Howard Hughes Medical Institute, Duke University Medical Center, Department of Medicine, Durham, NC 27710.

Kobilka T S

Yang-Feng T L

Caron M G

Lefkowitz R J

Kobilka B K

References (28)

28 references, click to expand

Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Adrenergic receptors. Models for the study of receptors coupled to guanine nucleotide regulatory proteins.
J Biol Chem. 1988 Apr 15;263(11):4993-6 PMID: 3128532
A functional basis for classification of alpha-adrenergic receptors.
Life Sci. 1977 Sep 1;21(5):595-606 PMID: 20542
Pharmacological classification of adrenergic alpha receptors in the guinea pig.
Nature. 1978 May 11;273(5658):164-6 PMID: 25390
Alpha-adrenoceptor subclassification.
Rev Physiol Biochem Pharmacol. 1981;88:199-236 PMID: 6259707
A simple method for displaying the hydropathic character of a protein.
J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
Validation and statistical analysis of a computer modeling method for quantitative analysis of radioligand binding data for mixtures of pharmacological receptor subtypes.
Mol Pharmacol. 1982 Jan;21(1):5-16 PMID: 6982395
[3H]Rauwolscine and [3H]yohimbine binding to rat cerebral and human platelet membranes: possible heterogeneity of alpha 2-adrenoceptors.
Eur J Pharmacol. 1982 Oct 15;84(1-2):79-85 PMID: 6291953
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Gene. 1982 Oct;19(3):259-68 PMID: 6295879
Isolation, sequence analysis, and intron-exon arrangement of the gene encoding bovine rhodopsin.
Cell. 1983 Oct;34(3):807-14 PMID: 6194890
Heterogeneity of alpha-2 adrenergic receptors.
Pharmacol Biochem Behav. 1985 May;22(5):835-43 PMID: 2989948
Purification and characterization of the human platelet alpha 2-adrenergic receptor.
J Biol Chem. 1986 Mar 15;261(8):3894-900 PMID: 3005306
Mammalian alpha 1-adrenergic receptor. Purification and characterization of the native receptor ligand binding subunit.
J Biol Chem. 1986 Jun 15;261(17):7710-6 PMID: 3011796
Alpha-2 adrenergic receptor subtypes indicated by [3H]yohimbine binding in human brain.
Life Sci. 1986 Jun 9;38(23):2129-37 PMID: 3012234
Photoaffinity labeling of human platelet and rabbit kidney alpha 2-adrenoceptors with [3H]SKF 102229.
Biochem Biophys Res Commun. 1986 Jun 13;137(2):606-13 PMID: 3015124
Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor.
Nature. 1986 Oct 2-8;323(6087):411-6 PMID: 3762692
cDNA for the human beta 2-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor.
Proc Natl Acad Sci U S A. 1987 Jan;84(1):46-50 PMID: 3025863
Primary structure and biochemical properties of an M2 muscarinic receptor.
Science. 1987 May 1;236(4801):600-5 PMID: 3107123
Anatomical evidence for alpha-2 adrenoceptor heterogeneity: differential autoradiographic distributions of [3H]rauwolscine and [3H]idazoxan in rat brain.
J Pharmacol Exp Ther. 1987 Jun;241(3):1079-91 PMID: 3037066
Pharmacological evidence for alpha-2 adrenoceptor heterogeneity: differential binding properties of [3H]rauwolscine and [3H]idazoxan in rat brain.
J Pharmacol Exp Ther. 1987 Jun;241(3):1092-8 PMID: 2885406
Identification of a family of muscarinic acetylcholine receptor genes.
Science. 1987 Jul 31;237(4814):527-32 PMID: 3037705
A family of receptors coupled to guanine nucleotide regulatory proteins.
Biochemistry. 1987 May 19;26(10):2657-64 PMID: 3038163
Use of eukaryotic expression technology in the functional analysis of cloned genes.
Methods Enzymol. 1987;152:684-704 PMID: 3657593
cDNA cloning of bovine substance-K receptor through oocyte expression system.
Nature. 1987 Oct 29-Nov 4;329(6142):836-8 PMID: 2823146
Cloning, sequencing, and expression of the gene coding for the human platelet alpha 2-adrenergic receptor.
Science. 1987 Oct 30;238(4827):650-6 PMID: 2823383
Functional activity and regulation of human beta 2-adrenergic receptors expressed in Xenopus oocytes.
J Biol Chem. 1987 Nov 15;262(32):15796-802 PMID: 2824467
Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors.
EMBO J. 1987 Dec 20;6(13):3923-9 PMID: 3443095
Presynaptic regulation of catecholamine release.
Biochem Pharmacol. 1974 Jul 1;23(13):1793-800 PMID: 4617579

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.85.17.6301

Published

1988-09-00

Pages

6301-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC281957

Subset

Databases

GENBANK

J03853

External Links

PubMed source DOI: 10.1073/pnas.85.17.6301 PMC Full Text View journal details

Analysis Services

Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype.

Contact

WeChat Official Account