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PMID: 2842299 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Staphylococcal enterotoxin B gene is associated with a discrete genetic element.

Journal of bacteriology ·Vol. 170 ·No. 9 ·1988-09-00 ·Pages 4033-9

Johns MB, Khan SA

Abstract

The chromosomal location of the enterotoxin B gene in Staphylococcus aureus is unknown. Southern hybridization analysis of the chromosomal DNA from several enterotoxin B (SEB)-producing strains has shown that at least 26.8 kilobases (kb) of DNA is associated with the enterotoxin B gene (entB). We have found that one end of the entB element is located approximately 1.5 kb downstream of the entB gene. The chromosomal region adjacent to this end of the entB element was found to be homologous in several SEB-producing (SEB+) and SEB-nonproducing (SEB-) S. aureus strains. The chromosomes of all the SEB+ strains studied were homologous for at least 24 kb upstream of the entB gene. Some naturally occurring SEB- strains lacked the entire entB element, while others showed variable homology to the region upstream of the entB gene. These data suggest that the entB gene is part of a discrete genetic element that is at least 26.8 kb in size.

MeSH Terms
Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/genetics Enterotoxins/genetics Genes, Bacterial Nucleic Acid Hybridization Staphylococcus aureus/genetics
Chemicals
DNA, Bacterial Enterotoxins enterotoxin B, staphylococcal DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Johns M B
Department of Microbiology, Biochemistry and Molecular Biology, University of Pittsburgh School of Medicine, Pennsylvania 15261.
Khan S A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.9.4033-4039.1988
Published
1988-09-00
Pages
4033-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211406
Subset
IM
Grants
NIAID NIH HHS · AI-19783 · United States
External Links
PubMed source DOI: 10.1128/jb.170.9.4033-4039.1988 PMC Full Text View journal details
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