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PMID: 4112070 Published · ppublish English Journal Article

Morphology and physical properties of Staphylococcus bacteriophage P11-M15.

Journal of virology ·Vol. 9 ·No. 4 ·1972-04-00 ·Pages 664-71

Brown DT, Brown NC, Burlingham BT

Abstract

The Group B Staphylococcus phage P11-M15 is shown to be 51% protein and 49% deoxyribonucleic acid (DNA). The intact virion has a molecular weight of 66.7 x 10(6) daltons. The purified viral DNA has a molecular weight of 32.7 x 10(6) daltons. The intact virion is shown to be composed of a polyhedral head which is attached at one of its vertices to a flexible tail having helical symmetry. The tail structure is terminated by a complex baseplate which has sixfold symmetry. The virion contains a single molecule of double-stranded DNA which has no apparent single-strand nicks or single-stranded terminal redundancies.

MeSH Terms
Centrifugation, Density Gradient Cesium Chlorides Chromatography, Ion Exchange DNA, Viral/analysis Freeze Etching Microscopy, Electron Models, Structural Molecular Weight Nucleic Acid Denaturation Phosphotungstic Acid Staining and Labeling Staphylococcus Staphylococcus Phages/analysis,isolation & purification Viral Proteins/analysis Virus Cultivation
Chemicals
Chlorides DNA, Viral Viral Proteins Phosphotungstic Acid Cesium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brown D T
Brown N C
Burlingham B T
References (18)
18 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
1972-04-00
Pages
664-71
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356352
Subset
IM
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