Abstract
The Group B Staphylococcus phage P11-M15 is shown to be 51% protein and 49% deoxyribonucleic acid (DNA). The intact virion has a molecular weight of 66.7 x 10(6) daltons. The purified viral DNA has a molecular weight of 32.7 x 10(6) daltons. The intact virion is shown to be composed of a polyhedral head which is attached at one of its vertices to a flexible tail having helical symmetry. The tail structure is terminated by a complex baseplate which has sixfold symmetry. The virion contains a single molecule of double-stranded DNA which has no apparent single-strand nicks or single-stranded terminal redundancies.
MeSH Terms
Centrifugation, Density Gradient
Cesium
Chlorides
Chromatography, Ion Exchange
DNA, Viral/analysis
Freeze Etching
Microscopy, Electron
Models, Structural
Molecular Weight
Nucleic Acid Denaturation
Phosphotungstic Acid
Staining and Labeling
Staphylococcus
Staphylococcus Phages/analysis,isolation & purification
Viral Proteins/analysis
Virus Cultivation
Chemicals
Chlorides
DNA, Viral
Viral Proteins
Phosphotungstic Acid
Cesium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brown D T
Brown N C
Burlingham B T
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18 references, click to expand
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