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PMID: 2840281 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Conservation of neural nicotinic acetylcholine receptors from Drosophila to vertebrate central nervous systems.

The EMBO journal ·Vol. 7 ·No. 3 ·1988-03-00 ·Pages 611-8

Bossy B, Ballivet M, Spierer P

Abstract

Nicotinic acetylcholine receptors (nAChR) are found both in vertebrate and insect central nervous systems. We have isolated a Drosophila gene by crosshybridization with a vertebrate probe. Structural conservation of domains of the deduced protein and of intron/exon boundaries indicate that the Drosophila gene encodes an nAChR alpha-like subunit (ALS). That the Drosophila gene product most resembles the neuronal set of vertebrate nAChRs alpha-subunits is also indicated by the failure of an ALS-beta-galactosidase fusion protein to bind alpha-bungarotoxin on blots in contrast to vertebrate endplate alpha-subunit constructions. The ALS encoding gene exceeds 54 kb in length and the transcript has a very long and unusual 5' leader. As we found previously for a gene whose product is also involved in cholinergic synapses, acetylcholinesterase, the leader encodes short open reading frames, which might be involved in translation control. We also note the presence of opa repeats in the gene, as has been found for various Drosophila genes expressed in the nervous system.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Bungarotoxins/metabolism Central Nervous System/metabolism,physiology Chromosomes/analysis DNA/analysis DNA Transposable Elements Drosophila/genetics,growth & development,metabolism Genes Molecular Sequence Data RNA, Messenger/metabolism Receptors, Nicotinic/genetics,metabolism,physiology Species Specificity Vertebrates/genetics,metabolism
Chemicals
Bungarotoxins DNA Transposable Elements RNA, Messenger Receptors, Nicotinic DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bossy B
Department of Molecular Biology, University of Geneva, Switzerland.
Ballivet M
Spierer P
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51 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-03-00
Pages
611-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454364
Subset
IM
Databases
GENBANK
X07194
External Links
PubMed source PMC Full Text View journal details
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