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PMID: 3037384 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family.

Nature ·Vol. 328 ·No. 6127 ·1987-00-00 ·Pages 221-7

Schofield PR, Darlison MG, Fujita N, Burt DR, Stephenson FA, Rodriguez H, Rhee LM, Ramachandran J, Reale V, Glencorse TA

Abstract

Amino-acid sequences derived from complementary DNAs encoding the alpha- and beta-subunits of the GABA/benzodiazepine receptor from bovine brain show homology with other ligand-gated receptor subunits, suggesting that there is a super-family of ion-channel-containing receptors. Co-expression of the in vitro-generated alpha-subunit and beta-subunit RNAs in Xenopus oocytes produces a functional receptor and ion channel with the pharmacological properties characteristic of the GABAA receptor.

MeSH Terms

Animals Cattle Cloning, Molecular DNA/genetics Gene Expression Regulation Membrane Proteins/genetics Multigene Family Oocytes/physiology Protein Conformation Receptors, GABA-A/genetics,physiology Receptors, Nicotinic/genetics gamma-Aminobutyric Acid/physiology

Chemicals

Membrane Proteins Receptors, GABA-A Receptors, Nicotinic gamma-Aminobutyric Acid DNA

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Schofield P R

Darlison M G

Fujita N

Burt D R

Stephenson F A

Rodriguez H

Rhee L M

Ramachandran J

Reale V

Glencorse T A

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/328221a0

Published

1987-00-00

Pages

221-7

Language

English

Region

England

NLM ID

0410462

Subset

Databases

GENBANK

X05717, X05718

External Links

PubMed source DOI: 10.1038/328221a0 View journal details

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Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family.

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