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PMID: 2837733 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence analysis of the NgoPII methyltransferase gene from Neisseria gonorrhoeae P9: homologies with other enzymes recognizing the sequence 5'-GGCC-3'.

Nucleic acids research ·Vol. 16 ·No. 10 ·1988-05-25 ·Pages 4369-87

Sullivan KM, Saunders JR

Abstract

Recombinant plasmids harbouring the functional M.NgoPII methyltransferase (specificity 5'-GGCC-3') were isolated from amplified gene libraries of gonococcal chromosomal DNA cloned in pBR322 and in Escherichia coli RR1. The M.NgoPII gene was localized by sub-cloning and the nucleotide sequence of a cloned 1.6 kb segment of Neisseria gonorrhoeae DNA harbouring the methylase gene was determined. This data, coupled with sub-cloning experiments and in vitro transcription-translation studies, indicates a theoretical size of 38.5 kd for the methylase protein. The predicted amino acid sequence of the methylase contains significant regions of homology with the projected sequences of other cytosine-modifying methylases, upon which the activity of these enzymes is likely to depend.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA-Cytosine Methylases Escherichia coli/genetics Genes Genes, Bacterial Methyltransferases/genetics Molecular Sequence Data Neisseria gonorrhoeae/enzymology,genetics Sequence Homology, Nucleic Acid
Chemicals
DNA-Cytosine Methylases Methyltransferases Cytosine 5-methyltransferase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sullivan K M
Department of Microbiology, University of Liverpool, UK.
Saunders J R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.10.4369
Published
1988-05-25
Pages
4369-87
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336636
Subset
IM
Grants
Wellcome Trust · United Kingdom
Databases
GENBANK
X06965
External Links
PubMed source DOI: 10.1093/nar/16.10.4369 PMC Full Text View journal details
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