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PMID: 2836437 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A molecular defect in virally transformed muscle cells that cannot cluster acetylcholine receptors.

The Journal of cell biology ·Vol. 106 ·No. 5 ·1988-05-00 ·Pages 1713-21

Anthony DT, Jacobs-Cohen RJ, Marazzi G, Rubin LL

Abstract

Muscle cells infected at the permissive temperature with temperature-sensitive mutants of Rous sarcoma virus and shifted to the non-permissive temperature form myotubes that are unable to cluster acetylcholine receptors (Anthony, D. T., S. M. Schuetze, and L. L. Rubin. 1984. Proc. Natl. Acad. Sci. USA. 81:2265-2269). Work described in this paper demonstrates that the virally-infected cells are missing a 37-kD peptide which reacts with an anti-tropomyosin antiserum. Using a monoclonal antibody specific for the missing peptide, we show that this tropomyosin is absent from fibroblasts and is distinct from smooth muscle tropomyosins. It is also different from the two previously identified striated muscle myofibrillar tropomyosins (alpha and beta). We suggest that, in normal muscle, this novel, non-myofibrillar, tropomyosin-like molecule is an important component of a cytoskeletal network necessary for cluster formation.

MeSH Terms
Animals Antibodies, Monoclonal Avian Sarcoma Viruses Cell Transformation, Viral Chickens Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Immunoassay Muscles/cytology Receptors, Cholinergic/metabolism,physiology Tropomyosin/analysis,immunology,physiology
Chemicals
Antibodies, Monoclonal Receptors, Cholinergic Tropomyosin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Anthony D T
Laboratory of Neurobiology, Rockefeller University, New York, New York 10021-6399.
Jacobs-Cohen R J
Marazzi G
Rubin L L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.106.5.1713
Published
1988-05-00
Pages
1713-21
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115064
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.106.5.1713 PMC Full Text View journal details
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