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PMID: 7026578 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cytoplasmic actin in postsynaptic structures at the neuromuscular junction.

The Journal of cell biology ·Vol. 90 ·No. 3 ·1981-09-00 ·Pages 789-92

Hall ZW, Lubit BW, Schwartz JH

Abstract

We used an antibody prepared against Aplysia (mollusc) body-wall actin that specifically reacts with certain forms of cytoplasmic actin in mammalian cells to probe for the presence of actin at the neuromuscular junction. Immunocytochemical studies showed that actin or an actinlike molecule is concentrated at neuromuscular junctions of normal and denervated adult rat muscle fibers. Actin is present at the neuromuscular junctions of fibers of developing diaphragm muscles as early as embryonic day 18, well before postsynaptic folds are formed. These results suggest that cytoplasmic actin may play a role in the clustering or stabilization of acetylcholine receptors at the neuromuscular junction.

MeSH Terms
Actins/analysis Animals Denervation Diaphragm/innervation Fluorescent Antibody Technique Motor Endplate/analysis,growth & development Neuromuscular Junction/analysis Rats Receptors, Cholinergic/analysis Synapses/analysis
Chemicals
Actins Receptors, Cholinergic
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hall Z W
Lubit B W
Schwartz J H
References (31)
31 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.90.3.789
Published
1981-09-00
Pages
789-92
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2111917
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.90.3.789 PMC Full Text View journal details
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