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PMID: 2836373 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA supercoiling and the anaerobic and growth phase regulation of tonB gene expression.

Journal of bacteriology ·Vol. 170 ·No. 6 ·1988-06-00 ·Pages 2816-26

Dorman CJ, Barr GC, Ni Bhriain N, Higgins CF

Abstract

We show that several interacting environmental factors influence the topology of intracellular DNA. Negative supercoiling of DNA in vivo is increased by anaerobic growth and is also influenced by growth phase. The tonB promoter of Escherichia coli and Salmonella typhimurium was found to be highly sensitive to changes in DNA supercoiling. Expression was increased by novobiocin, an inhibitor of DNA gyrase, and was decreased by factors which increase DNA superhelicity. Expression of the plasmid-encoded tonB gene was enhanced by gamma delta insertions in cis in a distance- and orientation-independent fashion. Both the res site and the TnpR protein of gamma delta, which is known to function as a type I topoisomerase, were required for this activation. tonB expression increased during the growth cycle and was reduced by anaerobiosis. There was excellent correlation between tonB expression from a plasmid and the level of supercoiling of that plasmid under a wide range of conditions. The chromosomal tonB gene was regulated in a manner identical to that of the plasmid-encoded gene. Thus, the physiological regulation of tonB expression in response to anaerobiosis and growth phase appears to be mediated by environmentally induced changes in DNA superhelicity.

MeSH Terms
Anaerobiosis Bacterial Proteins/genetics DNA, Superhelical/analysis Escherichia coli/genetics Gene Expression Regulation/drug effects Novobiocin/pharmacology Oxygen/pharmacology Plasmids Promoter Regions, Genetic Salmonella typhimurium/genetics
Chemicals
Bacterial Proteins DNA, Superhelical Novobiocin Oxygen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dorman C J
Department of Biochemistry, University of Dundee, Scotland.
Barr G C
Ni Bhriain N
Higgins C F
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.6.2816-2826.1988
Published
1988-06-00
Pages
2816-26
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211208
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.170.6.2816-2826.1988 PMC Full Text View journal details
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