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PMID: 6227609 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Regulation of enterobactin iron transport in Escherichia coli: characterization of ent::Mu d(Apr lac) operon fusions.

Journal of bacteriology ·Vol. 156 ·No. 3 ·1983-12-00 ·Pages 1171-7

Fleming TP, Nahlik MS, McIntosh MA

Abstract

The vector Mu d(Apr lac) was utilized to construct operon fusions in the Escherichia coli enterobactin (ent) biosynthetic and transport genes. Enzyme assays indicated a 5- to 15-fold increase in the expression of beta-galactosidase when the fusion strains were grown under iron-deficient conditions. The polarity effects seen by Mu d insertions into entA, entC, and entE were consistent with a single operon, entA(CGB)E. The direction of transcription from iron-regulated promoters was determined by directional transfer of selected genetic markers after the insertion of F'ts114 lac+. Regulatory mutants were isolated in the fusion strains by the selection for constitutive expression of beta-galactosidase and the iron-regulated outer membrane proteins.

MeSH Terms
Biological Transport DNA, Recombinant Enterobactin/biosynthesis,metabolism Escherichia coli/genetics,metabolism Gene Expression Regulation Genes, Bacterial Genes, Regulator Iron/metabolism Operon Serine/analogs & derivatives Transcription, Genetic
Chemicals
DNA, Recombinant Enterobactin Serine Iron
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fleming T P
Nahlik M S
McIntosh M A
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28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.156.3.1171-1177.1983
Published
1983-12-00
Pages
1171-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217964
Subset
IM
Grants
FDA HHS · BR5 5387 · United States
External Links
PubMed source DOI: 10.1128/jb.156.3.1171-1177.1983 PMC Full Text View journal details
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