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PMID: 2830457 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic analysis of a transposon carrying toluene degrading genes on a TOL plasmid pWW0.

Molecular & general genetics : MGG ·Vol. 210 ·No. 2 ·1987-12-00 ·Pages 270-6

Tsuda M, Iino T

Abstract

Toluene degrading (xyl) genes on a Pseudomonas TOL plasmid pWW0 are located within a 39-kb DNA portion. The 56-kb region including these xyl genes and its 17-kb derivative with a deletion of the internal 39-kb portion transposed to various sites on target replicons such as pACYC184 and R388 in Escherichia coli recA strains. Thus the 56- and 17-kb regions were designated Tn4651 and Tn4652, respectively. Genetic analysis of Tn4652 demonstrated that its transposition occurs by a two-step process, namely, cointegrate formation and its subsequent resolution. The presence in cis of DNA sequences of no more than 150 bp at both ends of Tn4652 was prerequisite for cointegrate formation, and this step was mediated by a trans-acting factor, transposase, which was encoded in a 3.0-kb segment at one end of the transposon. Cointegrate resolution took place site-specifically within a 200-bp fragment, which was situated 10 kb away from the transposase gene. Based on the stability of cointegrates formed by various mini-Tn4652 derivatives, it was shown that the cointegrate resolution requires two trans-acting factors encoded within 1.0- and 1.2-kb fragments that encompass the recombination site involved in the resolution.

MeSH Terms
Chromosome Deletion Chromosome Mapping DNA Transposable Elements Escherichia coli/genetics Genes, Bacterial Mutation Plasmids Pseudomonas/genetics Replicon Toluene/metabolism
Chemicals
DNA Transposable Elements Toluene
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tsuda M
Department of Biology, Faculty of Science, University of Tokyo, Japan.
Iino T
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33 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00325693
Published
1987-12-00
Pages
270-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00325693
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