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PMID: 418059 Published · ppublish English Journal Article

Isolation of TOL and RP4 recombinants by integrative suppression.

Journal of bacteriology ·Vol. 134 ·No. 1 ·1978-04-00 ·Pages 270-7

Nakazawa T, Hayashi E, Yokota T, Ebina Y, Nakazawa A

Abstract

We obtained genetic and molecular evidence of non-thermosensitive recombinants of RP4 (Kmr Tcr Cbr/Apr) and the thermosensitive TOL plasmid. As first isolated in Pseudomonas aeruginosa PAO, the recombinant plasmid pTN1 specified noninducible synthesis of TOL enzymes and was transmissible to Escherichia coli on selection for the transfer of kanamycin resistance. The phenotypic expression of TOL genes of pTN1 in E. coli was low and also noninducible. A spontaneous segregant, pTN2, appearing from pTN1, conferred inducible synthesis of TOL enzymes. These plasmids carry all of the TOL determinants as evidenced by the ability of Pseudomonas putida carrying recombinant plasmids to grow on toluene, xylene, and m-toluate. In E. coli the expression of TOL genes with normal regulation (pTN2) appears to be extremely low without induction, and the induced expression is comparable to that with defective regulation (pTN1). The measurement of the molecular weight of pTN2 by electron microscopy gave a value of about 74 X 10(6).

MeSH Terms
Conjugation, Genetic Escherichia coli/genetics Molecular Weight Plasmids Pseudomonas/genetics Pseudomonas aeruginosa/genetics,metabolism Recombination, Genetic Toluene/metabolism
Chemicals
Toluene
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nakazawa T
Hayashi E
Yokota T
Ebina Y
Nakazawa A
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.134.1.270-277.1978
Published
1978-04-00
Pages
270-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222243
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.134.1.270-277.1978 PMC Full Text View journal details
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