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PMID: 6792183 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of flagellar genes in Pseudomonas aeruginosa by use of Rfla plasmids and conjugations.

Journal of bacteriology ·Vol. 147 ·No. 3 ·1981-09-00 ·Pages 1008-14

Tsuda M, Oguchi T, Iino T

Abstract

Over 300 flagellar mutants were isolated in Pseudomonas aeruginosa PAO. R-prime plasmids carrying segments of bacterial chromosome which can complement the mutant phenotypes were isolated by means of plasmid R68.45. Among the R-prime plasmids, pMT6 complemented 167 out of 307 mutants examined, and pMT19 complemented the remaining 140 mutants. We found no mutant which was complemented by both of these plasmids. Hence, the flagellar genes were divided into two clusters by these two plasmids, namely, region I on pMT19 and region II on pMT6. By FP5- and R68.45-mediated conjugation, these two regions were located on the P. aeruginosa PAO chromosome with an order of puuF--region I--region II--oru-325.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial Conjugation, Genetic Flagella Genes, Bacterial Genetic Complementation Test Genetic Markers Mutation Pseudomonas aeruginosa/genetics,ultrastructure R Factors
Chemicals
Genetic Markers
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tsuda M
Oguchi T
Iino T
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.147.3.1008-1014.1981
Published
1981-09-00
Pages
1008-14
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216140
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.147.3.1008-1014.1981 PMC Full Text View journal details
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