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PMID: 408599 Published · ppublish English Journal Article

The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa.

Molecular & general genetics : MGG ·Vol. 154 ·No. 1 ·1977-07-07 ·Pages 7-22

Haas D, Holloway BW, Schamböck A, Leisinger T

Abstract

Six loci coding for arginine biosynthetic enzymes in Pseudomonas aeruginosa strain PAO were identified by enzyme assay: argA (N-acetylglutamate synthase), argB (N-acetylglutamate 5-phosphotransferase), argC (N-acetylglutamate 5-semialdehyde dehydrogenase), argF (anabolic ornithine carbamoyl-transferase), argG (argininosuccinate synthetase), and argH (argininosuccinase). One-step mutants which had a requirement for arginine and uracil were defective in carbamoylphosphate synthase, specified by a locus designated car. To map these mutations we used the sex factor FP2 in an improved interrupted mating technique as well as the generalized transducing phages F116L and G101. We confirmed earlier studies, and found no clustering of arg and car loci. However, argA, argH, and argB were mapped on a short chromosome segment (approx. 3 min long), and argF and argG were cotransducible, but not contiguous. N-Acetylglutamate synthase, the enzyme which replenishes the cycle of acetylated intermediates in ornithine synthesis of Pseudomonas, appears to be essential for arginine synthesis since argA mutants showed no growth on unsupplemented minimal medium.

MeSH Terms
Aldehyde Oxidoreductases/metabolism Arginine/biosynthesis Argininosuccinate Lyase/metabolism Argininosuccinate Synthase/metabolism Chromosome Mapping Chromosomes, Bacterial Conjugation, Genetic Genes Glutamate Synthase/metabolism Ornithine Carbamoyltransferase/metabolism Phosphate Acetyltransferase/metabolism Pseudomonas aeruginosa/enzymology Transduction, Genetic
Chemicals
Arginine Aldehyde Oxidoreductases Glutamate Synthase Ornithine Carbamoyltransferase Phosphate Acetyltransferase Argininosuccinate Lyase Argininosuccinate Synthase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Haas D
Holloway B W
Schamböck A
Leisinger T
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55 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00265571
Published
1977-07-07
Pages
7-22
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00265571
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