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PMID: 825027 Published · ppublish English Journal Article

Mutants of Pseudomonas aeruginosa that show specific hypersensitivity to aminoglycosides.

Antimicrobial agents and chemotherapy ·Vol. 10 ·No. 3 ·1976-09-00 ·Pages 411-6

Mills BJ, Holloway BW

Abstract

A class of mutants in Pseudomonas aeruginosa have been found that are tolerant to aeruginocin 41 and also hypersensitive to aminoglycosides. They do not show any changes in susceptibility to a wide range of other toxic agents, including antibiotics and surfactants. This tol locus, tolA, has been mapped at 10 min from the FP2 origin and linked to carA (carbamyl phosphate synthetase) by transduction and conjugation. By selecting for revertants of the hypersensitivity phenotype, revertants to tol(+) were found, indicating that it is the tolA locus that is responsible for this specific hypersensitivity. The results indicate that a specific mechanism exists for the intrinsic resistance of P. aeruginosa to aminoglycosides.

MeSH Terms
Aminoglycosides/pharmacology Anti-Bacterial Agents/pharmacology Bacteriocins/pharmacology Chromosome Mapping Conjugation, Genetic Drug Resistance, Microbial Microbial Sensitivity Tests Mutation Phenotype Pseudomonas aeruginosa/metabolism R Factors Selection, Genetic Streptomycin/metabolism Transduction, Genetic
Chemicals
Aminoglycosides Anti-Bacterial Agents Bacteriocins Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mills B J
Holloway B W
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21 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.10.3.411
Published
1976-09-00
Pages
411-6
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC429762
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.10.3.411 PMC Full Text View journal details
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