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PMID: 2827010 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

SRA5 encodes the low-Km cyclic AMP phosphodiesterase of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 8 ·No. 1 ·1988-01-00 ·Pages 505-10

Wilson RB, Tatchell K

Abstract

sra5 mutations in Saccharomyces cerevisiae were previously shown to suppress the inefficient growth of ras2 strains on nonfermentable carbon sources and to result in deficient low-Km cyclic AMP (cAMP) phosphodiesterase activity. We have cloned SRA5 by complementation. It maps to the right arm of chromosome XV, tightly linked to PRT1, and its sequence matches the sequence of PDE2, encoding the low-Km cAMP phosphodiesterase. Disruptions of SRA5 allowed ras1 ras2 strains to grow either on rich media supplemented with cAMP or on minimal media without exogenous cAMP. sra5 strains failed to survive prolonged nitrogen starvation in the presence of exogenous cAMP.

MeSH Terms
3',5'-Cyclic-AMP Phosphodiesterases/genetics Chromosome Mapping Cyclic AMP/physiology GTP-Binding Proteins/genetics Genes Genes, Fungal Genetic Complementation Test Mutation Nitrogen/metabolism Phenotype Saccharomyces cerevisiae/enzymology,genetics
Chemicals
Cyclic AMP 3',5'-Cyclic-AMP Phosphodiesterases GTP-Binding Proteins Nitrogen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wilson R B
Department of Biology, University of Pennsylvania, Philadelphia 19104.
Tatchell K
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31 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.1.505-510.1988
Published
1988-01-00
Pages
505-10
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363163
Subset
IM
Grants
NIGMS NIH HHS · 5-T32-GM-07170 · United States
NCI NIH HHS · CA37702 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.1.505-510.1988 PMC Full Text View journal details
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