Home LiteratureArticle Details
PMID: 2820935 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Physical mapping of transposon Tn5 insertions defines a gene cluster functional in nitrous oxide respiration by Pseudomonas stutzeri.

Journal of bacteriology ·Vol. 169 ·No. 10 ·1987-10-00 ·Pages 4577-80

Viebrock A, Zumft WG

Abstract

By transposon Tn5 mutagenesis, 19 strains of Pseudomonas stutzeri were acquired that had defects in nitrous oxide respiration (Nos- phenotype). A physical map of the mutants showed nearly random Tn5 insertions into genomic DNA within a single region ca. 8 kilobases long. Mutants were characterized immunochemically, enzymatically, and chemically. Several functions related to the synthesis and regulation of nitrous oxide reductase were associated with this DNA region, indicating that in P. stutzeri part of the genetic information necessary to respire nitrous oxide is clustered.

MeSH Terms
Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial/analysis,genetics Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Escherichia coli/genetics Genes, Bacterial Mutation Nitrous Oxide/metabolism Oxidoreductases/biosynthesis,genetics Phenotype Pseudomonas/enzymology,genetics,metabolism
Chemicals
DNA Transposable Elements DNA, Bacterial Oxidoreductases nitrous oxide reductase DNA Restriction Enzymes Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Nitrous Oxide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Viebrock A
Lehrstuhl für Mikrobiologie, Universität Karlsruhe, Federal Republic of Germany.
Zumft W G
References (31)
31 references, click to expand
  1. A new spectrophotometric assay for protein in cell extracts.
    Anal Biochem. 1977 Oct;82(2):362-71 PMID: 20815
  2. Nutritional requirements of the predominant rumen cellulolytic bacteria.
    Fed Proc. 1973 Jul;32(7):1809-13 PMID: 4718898
  3. Purification and properties of an exo-cellulase of Avicelase type from a wood-rotting fungus, Irpex lacteus (Polyporus tulipiferae).
    J Biochem. 1978 Nov;84(5):1217-26 PMID: 32175
  4. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
    Gene. 1979 May;6(1):23-8 PMID: 383576
  5. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  6. In vitro packaging of lambda and cosmid DNA.
    Methods Enzymol. 1979;68:299-309 PMID: 161604
  7. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.
    Science. 1981 Mar 27;211(4489):1437-8 PMID: 6162199
  8. Cellulolytic enzyme system of Acetivibrio cellulolyticus.
    Can J Microbiol. 1981 Mar;27(3):288-94 PMID: 6786718
  9. Features of rumen and sewage sludge strains of Eubacterium limosum, a methanol- and H2-CO2-utilizing species.
    Appl Environ Microbiol. 1981 Jul;42(1):12-9 PMID: 6791591
  10. Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis.
    J Bacteriol. 1982 Jan;149(1):114-22 PMID: 6274841
  11. Purification and properties of cellobiosidase from Ruminococcus albus.
    J Bacteriol. 1982 Apr;150(1):407-9 PMID: 7061402
  12. An assay for selective determination of exo-1,4,-beta-glucanases in a mixture of cellulolytic enzymes.
    Anal Biochem. 1984 May 1;138(2):481-7 PMID: 6430117
  13. Purification and characterization of an exoglucanase from Streptomyces flavogriseus.
    Can J Microbiol. 1984 Sep;30(9):1171-8 PMID: 6509395
  14. Isolation and properties of beta-glucosidase from Ruminococcus albus.
    J Bacteriol. 1985 Jan;161(1):432-4 PMID: 3918007
  15. Fermentation of cellodextrins by cellulolytic and noncellulolytic rumen bacteria.
    Appl Environ Microbiol. 1985 Mar;49(3):572-6 PMID: 3994365
  16. Isolation and characterization of transposon Tn5-induced mutants of Pseudomonas perfectomarina defective in nitrous oxide respiration.
    J Bacteriol. 1985 Sep;163(3):918-24 PMID: 2993252
  17. Nitrous oxide reductase from denitrifying Pseudomonas perfectomarina. Purification and properties of a novel multicopper enzyme.
    Eur J Biochem. 1985 Dec 16;153(3):459-67 PMID: 3000778
  18. Inability of Pseudomonas stutzeri denitrification mutants with the phenotype of Pseudomonas aeruginosa to grow in nitrous oxide.
    Appl Environ Microbiol. 1985 Nov;50(5):1301-3 PMID: 3937493
  19. THE CELLULOLYTIC ACTIVITY OF PURE STRAINS OF BACTERIA FROM THE RUMEN OF CATTLE.
    J Gen Microbiol. 1963 Sep;32:441-8 PMID: 14058994
  20. A submicrodetermination of glucose.
    J Biol Chem. 1949 Nov;181(1):149-51 PMID: 15390401
  21. The anaerobic mesophilic cellulolytic bacteria.
    Bacteriol Rev. 1950 Mar;14(1):1-49 PMID: 15420122
  22. The cellulase of Trichoderma viride. Separation of the components involved in the solubilization of cotton.
    Biochem J. 1967 Sep;104(3):716-24 PMID: 6069203
  23. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  24. Kinetic studies of the action of cellulase upon sodium carboxymethyl cellulose.
    Arch Biochem Biophys. 1969 Sep;133(2):233-7 PMID: 5387561
  25. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  26. The purification and properties of extracellular beta-glucosidase from Botryodiplodia theobromae Pat.
    Biochim Biophys Acta. 1971 Feb 10;227(2):419-28 PMID: 5550826
  27. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
    Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
  28. Alcian blue staining of glycoproteins.
    Anal Biochem. 1972 Aug;48(2):621-3 PMID: 4115986
  29. Commentary on the Hungate technique for culture of anaerobic bacteria.
    Am J Clin Nutr. 1972 Dec;25(12):1324-8 PMID: 4565349
  30. The purification and properties of the C 1 component of Trichoderma koningii cellulase.
    Biochem J. 1972 Aug;128(5):1183-92 PMID: 4674624
  31. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.10.4577-4580.1987
Published
1987-10-00
Pages
4577-80
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213824
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.10.4577-4580.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2