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PMID: 3918007 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and properties of beta-glucosidase from Ruminococcus albus.

Journal of bacteriology ·Vol. 161 ·No. 1 ·1985-01-00 ·Pages 432-4

Ohmiya K, Shirai M, Kurachi Y, Shimizu S

Abstract

An enzyme active against p-nitrophenyl-beta-D-glucoside was purified from logarithmic-phase cells of Ruminococcus albus cultivated in a medium containing ball-milled cellulose. The purification yielded homogeneous enzyme after an approximately 520-fold increase in specific activity and a 9% yield. The enzyme was identified as a beta-glucosidase because it can hydrolyze cellobiose and cellooligosaccharides to glucose from the nonreducing ends.

MeSH Terms

Cations, Divalent Cellobiose/metabolism Enzyme Activation Glucose/metabolism Glucosidases/metabolism Glucosides/metabolism Kinetics Molecular Weight Peptococcaceae/enzymology beta-Glucosidase/isolation & purification,metabolism

Chemicals

Cations, Divalent Glucosides Cellobiose 4-nitrophenyl beta-D-glucoside 4-nitrophenyl beta-cellobioside Glucosidases beta-Glucosidase Glucose

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ohmiya K

Shirai M

Kurachi Y

Shimizu S

References (9)

9 references, click to expand

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J Bacteriol. 1982 Apr;150(1):407-9 PMID: 7061402
Cellulolytic activity of the rumen bacterium Bacteroides succinogenes.
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Hydrophobic interaction chromatography on uncharged Sepharose derivatives. Effects of neutral salts on the adsorption of proteins.
J Chromatogr. 1977 Jan 21;131:99-108 PMID: 853109
Comparison of Extracellular Cellulase Activities of Clostridium thermocellum LQRI and Trichoderma reesei QM9414.
Appl Environ Microbiol. 1981 Aug;42(2):231-40 PMID: 16345823
Rumen bacterial degradation of forage cell walls investigated by electron microscopy.
Appl Microbiol. 1975 May;29(5):692-701 PMID: 16350017
The mechanism of enzymatic cellulose degradation. Isolation and some properties of a beta-glucosidase from Trichoderma viride.
Eur J Biochem. 1974 Jul 15;46(2):295-305 PMID: 4854948
Cellulose degradation by Ruminococcus.
Fed Proc. 1973 Jul;32(7):1814-8 PMID: 4718899
Enzymatic studies of pure cultures of rumen microorganisms.
J Bacteriol. 1966 Nov;92(5):1321-30 PMID: 4380801
Purification and characterization of an endoglucanase (1,4-beta-D-glucan glucanohydrolase) from Clostridium thermocellum.
Biochem J. 1981 Nov 1;199(2):341-50 PMID: 7340808

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.161.1.432-434.1985

Published

1985-01-00

Pages

432-4

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC214890

Subset

External Links

PubMed source DOI: 10.1128/jb.161.1.432-434.1985 PMC Full Text View journal details

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Isolation and properties of beta-glucosidase from Ruminococcus albus.

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