Purification and properties of cellobiosidase from Ruminococcus albus.

Journal of bacteriology ·Vol. 150 ·No. 1 ·1982-04-00 ·Pages 407-9

Ohmiya K, Shimizu M, Taya M, Shimizu S

Abstract

An enzyme active against p-nitrophenyl-beta-d-cellobioside was purified from Ruminococcus albus to homogeneity. The enzyme was identified as a cellobiosidase.

MeSH Terms

Glucosides/metabolism Glycoside Hydrolases/isolation & purification,metabolism Isoelectric Point Kinetics Molecular Weight Peptococcaceae/enzymology

Chemicals

Glucosides 4-nitrophenyl beta-cellobioside Glycoside Hydrolases cellobiosidase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ohmiya K

Shimizu M

Taya M

Shimizu S

References (5)

5 references, click to expand

The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Microbial sources of cellulase.
Biotechnol Bioeng Symp. 1975;(5):81-105 PMID: 98
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Notes on sugar determination.
J Biol Chem. 1952 Mar;195(1):19-23 PMID: 14938350
The biological degradation of soluble cellulose derivatives and its relationship to the mechanism of cellulose hydrolysis.
J Bacteriol. 1950 Apr;59(4):485-97 PMID: 15436422

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.150.1.407-409.1982

Published

1982-04-00

Pages

407-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC220130

Subset

External Links

PubMed source DOI: 10.1128/jb.150.1.407-409.1982 PMC Full Text View journal details

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Purification and properties of cellobiosidase from Ruminococcus albus.

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