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PMID: 2808531 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Microinjection of a monoclonal antibody against a 37-kD protein (tropomyosin 2) prevents the formation of new acetylcholine receptor clusters.

The Journal of cell biology ·Vol. 109 ·No. 5 ·1989-11-00 ·Pages 2337-44

Marazzi G, Bard F, Klymkowsky MW, Rubin LL

Abstract

We have shown previously that chick muscle cells transformed with Rous sarcoma virus are unable to form clusters of acetylcholine receptors (AChRs) (Anthony, D. T., S. M. Schuetze, and L. L. Rubin. 1984. Proc. Natl. Acad. Sci. USA. 81:2265-2269) and are missing a 37-KD tropomyosin-like protein (TM-2) (Anthony, D. T., R. J. Jacobs-Cohen, G. Marazzi, and L. L. Rubin. 1988. J. Cell Biol. 106:1713-1721). In an attempt to clarify the role of TM-2 in the formation and/or maintenance of AChR clusters, we have microinjected a monoclonal antibody specific for TM-2 (D3-16) into normal chick muscle cells in culture. D3-16 injection blocks the formation of new clusters but does not affect the preexisting ones. In addition, TM-2 is concentrated at rat neuromuscular junctions. These data suggest that TM-2 may play an important role in promoting the formation of AChR clusters.

MeSH Terms
Animals Antibodies, Monoclonal/administration & dosage Azides/pharmacology Chick Embryo Microinjections Muscles/cytology,drug effects,physiology Neuromuscular Junction/physiology Receptors, Cholinergic/physiology Sodium Azide Tropomyosin/immunology,physiology
Chemicals
Antibodies, Monoclonal Azides Receptors, Cholinergic Tropomyosin Sodium Azide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Marazzi G
Laboratory of Neurobiology, Rockefeller University, New York 10021.
Bard F
Klymkowsky M W
Rubin L L
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28 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.109.5.2337
Published
1989-11-00
Pages
2337-44
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115874
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.109.5.2337 PMC Full Text View journal details
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