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PMID: 2785916 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rapid induction of transcription of unrearranged S gamma 1 switch regions in activated murine B cells by interleukin 4.

The EMBO journal ·Vol. 8 ·No. 2 ·1989-02-00 ·Pages 483-8

Esser C, Radbruch A

Abstract

The distribution of immunoglobulin isotypes in activated B lymphocytes can be modulated by interleukin 4 (IL4), which enhances IgG1 and suppresses IgG3. We show here that IL4 induces transcription of the region 5' adjacent to the s gamma 1 switch region within hours after onset of activation of B cells by bacterial lipopolysaccharide (LPS). Transcripts of 1.7 and 3.2 kb size containing sequences of the region 5' of s gamma 1 are detected. This transcription precedes class switch recombination between s mu and s gamma 1 and reflects the rapid opening of the s gamma 1 region as induced by IL4. This suggests that IL4 directs class switching to IgG1 by opening the s gamma 1 switch region, thus making it accessible for switch recombination.

MeSH Terms
Animals B-Lymphocytes/immunology,metabolism Female Genes, Immunoglobulin/drug effects Genes, Regulator/drug effects Genes, Switch/drug effects Immunoglobulin G/biosynthesis,genetics In Vitro Techniques Interleukin-4 Interleukins/pharmacology Lipopolysaccharides/pharmacology Lymphocyte Activation Mice Mice, Inbred Strains Transcription, Genetic/drug effects
Chemicals
Immunoglobulin G Interleukins Lipopolysaccharides Interleukin-4
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Esser C
Institut für Genetik der Universität zu Köln, FRG.
Radbruch A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-02-00
Pages
483-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400830
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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