Home LiteratureArticle Details
PMID: 6439816 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s).

The Journal of experimental medicine ·Vol. 160 ·No. 6 ·1984-12-01 ·Pages 1850-63

Layton JE, Vitetta ES, Uhr JW, Krammer PH

Abstract

To gain insight into how T cell-derived lymphokines induce the secretion of IgG in activated B cells, we performed a limiting dilution analysis, using murine splenic B cells incubated with lipopolysaccharide (LPS) and a T cell-derived B cell differentiating factor for IgG (BCDF gamma)-containing supernatant (SN). The results of this analysis indicate that such a SN induces a marked increase in the precursor frequency of IgG1-secreting cells and a modest increase in clone size. The precursors lack surface IgG and are committed to the differentiation pathway for IgG1 secretion after LPS activation, but before the addition of BCDF gamma-containing SN. The majority of IgG1-secreting clones arise independently from precursors of cells that secrete IgG3. Taken together, these results indicate that BCDF gamma directs differentiation of activated B cells to IgG1 secretion.

MeSH Terms
Animals Antigens, Differentiation, B-Lymphocyte Antigens, Surface/pharmacology B-Lymphocytes/immunology Clone Cells/immunology Female Immunoglobulin G/metabolism Lipopolysaccharides/pharmacology Lymphokines/pharmacology Mice Mice, Inbred C57BL Mice, Inbred DBA Rats Rats, Inbred Lew T-Lymphocytes/metabolism
Chemicals
Antigens, Differentiation, B-Lymphocyte Antigens, Surface Immunoglobulin G Lipopolysaccharides Lymphokines
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Layton J E
Vitetta E S
Uhr J W
Krammer P H
References (30)
30 references, click to expand
  1. Thymus dependence of antibody response: variation with dose of antigen and class of antibody.
    Nature. 1968 Nov 30;220(5170):927-8 PMID: 5722148
  2. Regulation of Ig class secretion by soluble products of certain T-cell lines.
    Immunol Rev. 1984 Apr;78:25-50 PMID: 6234222
  3. Regulatory role of T cells in IgG antibody formation and immune memory to type III Pneumococcal polysaccharide.
    J Immunol. 1974 Dec;113(6):1909-20 PMID: 4154347
  4. Immunologic and physicochemical properties of enhancing soluble factors for IgG and IgE antibody responses.
    J Immunol. 1975 Apr;114(4):1177-84 PMID: 1167880
  5. B lymphocyte differentiation induced by lipopolysaccharide. I. Generation of cells synthesizing four major immunoglobulin classes.
    J Immunol. 1975 Sep;115(3):671-6 PMID: 1097525
  6. A plaque assay for all cells secreting Ig of a given type or class.
    Eur J Immunol. 1976 Aug;6(8):588-90 PMID: 789096
  7. Conjugation of antibodies with fluorochromes: modifications to the standard methods.
    J Immunol Methods. 1976;13(3-4):215-26 PMID: 796385
  8. Surface immunoglobulins of lipopolysaccharide-stimulated spleen cells. The behavior of IgM, IgD and IgG.
    Eur J Immunol. 1977 Mar;7(3):151-3 PMID: 301090
  9. Organization of immunoglobulin heavy chain genes and allelic deletion model.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2140-4 PMID: 97665
  10. The switch from IgM to IgG secretion in single mitogen-stimulated B-cell clones.
    J Exp Med. 1978 Jun 1;147(6):1744-54 PMID: 308089
  11. Ability of antigen-specific helper cells to effect a class-restricted increase in total Ig-secreting cells in spleens after immunization with the antigen.
    J Exp Med. 1979 Sep 19;150(3):517-30 PMID: 90107
  12. The role of gene deletion in the immunoglobulin heavy chain switch.
    Nature. 1980 Jan 24;283(5745):351-6 PMID: 6766206
  13. Subclass restriction of murine antibodies. II. The IgG plaque-forming cell response to thymus-independent type 1 and type 2 antigens in normal mice and mice expressing an X-linked immunodeficiency.
    J Exp Med. 1980 Apr 1;151(4):853-62 PMID: 6966310
  14. The effect of anti-immunoglobulin antibodies on the in vitro proliferation and differentiation of normal and neoplastic murineB cells.
    J Immunol. 1980 Aug;125(2):886-92 PMID: 6771332
  15. An in vitro line of the B cell tumor BCL1 can be activated by LPS to secrete IgM1.
    J Immunol. 1980 Sep;125(3):976-80 PMID: 6157730
  16. Deletion of immunoglobulin heavy chain genes from expressed allelic chromosome.
    Nature. 1980 Aug 28;286(5776):850-3 PMID: 6774261
  17. T cell regulation of IgG subclass antibody production in response to T-independent antigens.
    J Exp Med. 1981 Jan 1;153(1):1-12 PMID: 6969777
  18. Expression of surface and secreted IgG2a by a murine B-lymphoma before and after hybridization to myeloma cells.
    Mol Immunol. 1981 Apr;18(4):311-22 PMID: 6795453
  19. T cell-derived B cell differentiation factor(s). Effect on the isotype switch of murine B cells.
    J Exp Med. 1982 Mar 1;155(3):734-48 PMID: 7038025
  20. A simple semi-automated plaque method for the detection of antibody-forming cell clones in microcultures.
    J Immunol Methods. 1982;52(1):25-37 PMID: 6749990
  21. Rearrangements between the immunoglobulin heavy chain gene JH and C mu regions accompany normal B lymphocyte differentiation in vitro.
    Nature. 1982 Oct 21;299(5885):742-4 PMID: 6811953
  22. Human T cell hybridomas secreting factors for IgA-specific help, polyclonal B cell activation, and B cell proliferation.
    J Exp Med. 1982 Dec 1;156(6):1860-5 PMID: 6983562
  23. Mechanisms regulating IgA class-specific immunoglobulin production in murine gut-associated lymphoid tissues. I. T cells derived from Peyer's patches that switch sIgM B cells to sIgA B cells in vitro.
    J Exp Med. 1983 Feb 1;157(2):433-50 PMID: 6185611
  24. T cell-derived B cell growth and differentiation factors. Dichotomy between the responsiveness of B cells from adult and neonatal mice.
    J Exp Med. 1983 Feb 1;157(2):600-12 PMID: 6600488
  25. Immunoglobulin C gene expression. IV. Alternative control of IgG1-producing cells by helper cell-derived B cell-specific growth or maturation factors.
    Eur J Immunol. 1983 Mar;13(3):269-72 PMID: 6219885
  26. Mechanisms regulating IgA class-specific immunoglobulin production in murine gut-associated lymphoid tissues. II. Terminal differentiation of postswitch sIgA-bearing Peyer's patch B cells.
    J Exp Med. 1983 Sep 1;158(3):649-69 PMID: 6604126
  27. Deletion of Cmu genes in mouse B lymphocytes upon stimulation with LPS.
    EMBO J. 1983;2(11):1929-35 PMID: 6315407
  28. Effect of T cell-derived lymphokines containing B cell differentiation factor(s) for IgG (BCDF gamma) on gamma-specific mRNA in murine B cells.
    J Immunol. 1983 Dec;131(6):3049-51 PMID: 6605997
  29. T-cell-derived lymphokines that induce IgM and IgG secretion in activated murine B cells.
    Immunol Rev. 1984 Apr;78:137-57 PMID: 6376330
  30. Replacement of T-cell function by a T-cell product.
    Nat New Biol. 1972 May 3;237(70):15-7 PMID: 4503682
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.160.6.1850
Published
1984-12-01
Pages
1850-63
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187537
Subset
IM
Grants
NIAID NIH HHS · AI-11851 · United States
External Links
PubMed source DOI: 10.1084/jem.160.6.1850 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2