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PMID: 6966310 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Subclass restriction of murine antibodies. II. The IgG plaque-forming cell response to thymus-independent type 1 and type 2 antigens in normal mice and mice expressing an X-linked immunodeficiency.

The Journal of experimental medicine ·Vol. 151 ·No. 4 ·1980-04-01 ·Pages 853-62

Slack J, Der-Balian GP, Nahm M, Davie JM

Abstract

Antigens have been classified previously into three categories, thymus-dependent (TD), thymus-independent type (TI) 1, and TI-2, based upon thymic dependence and ability to stimulate an immunodeficient strain of mouse, CBA/N. Here we demonstrate that the different antigen classes elicit IgG antibodies of different subclasses. TD antigens stimulate predominantly IgG1 antibodies, with smaller amounts of IgG2 and IgG3 being expressed. TI-1 antigens stimulate almost no IgG1 antibodies and equal amounts of IgG2 and IgG3. TI-2 antigens elicit predominantly IgG3 antibodies. Mice expressing the CBA/N phenotype are known to be nonresponsive to TI-2 antigens. This was confirmed in this study. In addition, we demonstrate that the IgG3 component of the response to TI-1 antigens is virtually absent in mice expressing the CBA/N phenotype, which supports our previous finding that the CBA/N defect may be restricted to a B-lymphocyte subpopulation containing most of the precursors of IgG3-secreting cells.

MeSH Terms
Animals Antibody Formation B-Lymphocytes/immunology Female Ficoll/immunology Genetic Linkage Immunoglobulin G/biosynthesis Immunoglobulin M/biosynthesis Immunologic Deficiency Syndromes/genetics,immunology Lipopolysaccharides/immunology Mice Mice, Inbred CBA/immunology T-Lymphocytes/immunology X Chromosome
Chemicals
Immunoglobulin G Immunoglobulin M Lipopolysaccharides Ficoll
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Slack J
Der-Balian G P
Nahm M
Davie J M
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41 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.151.4.853
Published
1980-04-01
Pages
853-62
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2185832
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.151.4.853 PMC Full Text View journal details
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