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PMID: 3086872 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Class switch recombination is IgG1 specific on active and inactive IgH loci of IgG1-secreting B-cell blasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 11 ·1986-06-00 ·Pages 3954-7

Radbruch A, Müller W, Rajewsky K

Abstract

Mouse B lymphocytes can be activated polyclonally by bacterial lipopolysaccharide (LPS) to secrete Ig and perform Ig class switch. In the presence of the T-cell lymphokine B-cell differentiation factor, the frequency of IgG1-secreting cells is drastically enhanced. We show here that IgG1-secreting B cells isolated from such cultures have undergone a similar DNA rearrangement of the switch regions (S mu, S gamma 1) of the Ig heavy chain constant region genes C mu and C gamma 1 on both active and inactive IgH loci. This result argues against a stochastic model of class switch recombination and suggests programmed class-specific switch recombination in the case of the switch to IgG1. In accord with this notion, cells expressing IgM but not IgG on the surface have not deleted or rearranged C mu or S gamma 1 on either chromosome.

MeSH Terms
Animals Antigens, Differentiation, B-Lymphocyte Antigens, Surface/physiology B-Lymphocytes/physiology Gene Expression Regulation Genes Immunoglobulin Constant Regions/genetics Immunoglobulin Heavy Chains/genetics Immunoglobulin gamma-Chains/classification,genetics Immunoglobulin mu-Chains/genetics Lymphocyte Activation Mice Recombination, Genetic
Chemicals
Antigens, Differentiation, B-Lymphocyte Antigens, Surface Immunoglobulin Constant Regions Immunoglobulin Heavy Chains Immunoglobulin gamma-Chains Immunoglobulin mu-Chains
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Radbruch A
Müller W
Rajewsky K
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.11.3954
Published
1986-06-00
Pages
3954-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323643
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.11.3954 PMC Full Text View journal details
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